In vivo antigen expression by Pasteurella multocida. |
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Authors: | J R Glisson I H Cheng |
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Institution: | Department of Avian Medicine, University of Georgia, Athens 30602. |
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Abstract: | Pasteurella multocida was purified from the blood of turkeys affected with acute fowl cholera, and membrane preparations from those bacteria were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized on immunoblots. Antigens were detected in the membranes of these in vivo-propagated bacteria that were not detected in membrane preparations of the same P. multocida strain grown in vitro. The unique antigens were detected in the detergent-insoluble phase and were enriched to various degrees by different detergents. |
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