低温胁迫对ICE1转基因水稻苗期抗氧化系统的影响

试验以水稻垦鉴稻10号及其转ICE1（Inducer of CBF expression 1）基因水稻T2-9株系为实验材料,低温（0℃）处理水稻幼苗0-10 d,测定抗氧化酶（SOD、CAT、POD、GR和APX）活性。试验结果表明,常温下,转基因植株抗氧化酶活性与对照没有显著差异;经低温胁迫后,转基因水稻和非转基因水稻的抗氧化酶活性在变化幅度方面有明显的差异。冷处理12h后,除POD外非转基因水稻的抗氧化酶活性开始下降,而转基因水稻酶活性仍继续升高;SOD、CAT、APX和GR活性分别在冷处理2、4、4、6 d达到最大值。与非转基因水稻相比,低温胁迫过程中转基因水稻POD活性没有明显变化。非转基因水稻POD活性水平则明显升高,在冷处理2天时达到最大值,是同处理时期转基因水稻POD活性的2.1倍。

The effect of low temperature stress to the antioxidant system of the ICE1 transgenic rice at seeding stage

The rice （Oryza sativa L. cv Kenjiandao No.10） and its ICE1 transgenic line T2-9 was used as the material of this experiment. The seedlings of rice were treated with low temperature （0 ~C ） stress, for 1-10 days, and the activity of antioxidant enzyme （SOD, CAT, POD, GR and APX） were determined. The results indicated that the activity of antioxidant enzyme did not show significant different between the transgenic rice and the control at normal temperature, After low temperature, the activity level of antioxidant enzyme in transgenic rice was significant different from that of non-transgenic rice. Enhancement of antioxidant enzyme activity was obtained in transgenic rice except POD, but reduction occurred in non-transgenic rice after 12 hours of cold stress. POD activity of transgenie rice is not apparently diversity compared with non-transgenic rice under low temperature. However, the activity of POD was higher in control than in transgenie rice, and reached to the highest in treated 2 days which was 2.1 times than transgenic rice＇ s.