| 油桐DGAT2基因克隆及其RNAi双元表达载体构建 |
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| 引用本文： | 徐玲娜,汪阳东,陈益存,张姗姗. 油桐DGAT2基因克隆及其RNAi双元表达载体构建[J]. 林业科学研究, 2011, 24(5): 668-673 |
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| 作者姓名： | 徐玲娜 汪阳东 陈益存 张姗姗 |
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| 作者单位： | 1. 中国林业科学研究院亚热带林业研究所,浙江富阳311400;安徽师范大学生命科学学院,安徽芜湖241000
2. 中国林业科学研究院亚热带林业研究所,浙江富阳,311400 |
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| 基金项目： | 国家林业局"948"引进项目"木本油料植物油脂合成基因高通量分析技术引进"(2009-4-23) |
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| 摘 要： |
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| 关 键 词： | 油桐 DGAT2 RNAi 双向启动子 |
| 收稿时间： | 2010-11-26 |
Cloning of Tung Tree DGAT2 Gene and Construction of RNAi Binary Expression Vector with Convergent Promoters |
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| XU Ling-n,WANG Yang-dong,CHEN Yi-cun and ZHANG Shan-shan. Cloning of Tung Tree DGAT2 Gene and Construction of RNAi Binary Expression Vector with Convergent Promoters[J]. Forest Research, 2011, 24(5): 668-673 |
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| Authors: | XU Ling-n WANG Yang-dong CHEN Yi-cun ZHANG Shan-shan |
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| Affiliation: | Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;College of Life Science, Anhui Normal University, Wuhu 241000, Anhui, China;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Fuyang 311400, Zhejiang, China;College of Life Science, Anhui Normal University, Wuhu 241000, Anhui, China |
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| Abstract: | To identify the physiological function of Diacylglycerol Acyltransferase 2 (DGAT2) coding gene in the process of tung oil biosynthesis, DGAT2 was cloned from cDNA of tung tree kernel and then linked with pMD18-T vector for sequencing. The 969bp fragment containing Open Reading Frame was acquired. Subsequently, RNAi binary expression vector pD35-DGAT2 was constructed, which expressed DGAT2 in two opposite ways. The studies provide the possibilities to further identify the function of DGAT2 in tung oil biosynthesis by RNAi technology and hold promise for genetic engineering of Venicia fordii. |
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| Keywords: | tung tree DGAT2 RNAi convergent promoters |
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