抗稻瘟病单基因系对籼稻稻瘟病菌小种鉴别力分析

 利用30个抗稻瘟病单基因系对146个来自广东的稻瘟病菌单孢分离菌株进行了致病性测定,其中17个单基因系对测试菌株的致病性有较好的鉴别力。基于主成分因子分析法,对17个单基因系和146个菌株组成的互作变量矩阵进行了因子提取,结果表明其中9个单基因系为代表的抽提因子可以解释变量总方差的80.437%,初步组建了这9个单基因系构成的稻瘟病菌生理小种单基因系鉴别寄主。按其对变量方差贡献大小排序,它们分别是:IRBLkp-K 60(Pi-k^p)、IRBLi-F5(Pi-i)、F128-1(Pi-ta²)、IRBL9-W (Pi-9(t))、IRBLsh-S (Pi-sh(1))、IRBLz-Fu (Pi-z)、IRBLkh-K3(Pi-k^h)、IRBL1-CL (Pi-1)、IRBLz5-CA (Pi-z⁵)。用这9个单基因系可将146个菌株划分为83个小种,将来自广西、福建、湖南、湖北、浙江、四川等籼稻区的54个菌株划分为26个小种。筛选的9个单基因系对我国籼稻区稻瘟病菌致病性有较强的鉴别力。在抗病性鉴定中,基于单基因系的代表菌株选择,可考虑应用聚类和变量分析法进行。

Differentiation ability of monogenic lines to Magnaporthe grisea in indica rice

Pathogenicity of 146 Magnaporthe grisea single spore isolates from indica rice in Guangdong was analyzed against 30 rice blast monogenic lines. Seventeen lines were selected to be the candidates for further construction of differentials. Factor analysis with principal component method of the variance matrix based on the interaction between 17 monogenic lines and 146 blast isolates had been conducted. Nine monogenic lines explaining 80.437% of the total variance were abstracted. According to their contribution to the percentage of variance, they were listed as follows: IRBLkp-K60(Pi-k~p), IRBLi-F5(Pi-i), F128-1(Pi-ta~2), IRBL9-W(Pi-9(t)), IRBLsh-S(Pi-sh(1)), IRBLz-Fu(Pi-z), IRBLkh-K3(Pi-k~h), IRBL1-CL(Pi-1) and IRBLz5-CA(Pi-z~5).Those lines were recommended to be blast monogenic dif-(ferentials) with which 83 races were identified among the 146 Guangdong isolates and 26 races among 54 (isolates) from indica rice cultivars in other six provinces. The results showed that 9 selected monogenic lines had good abilities for differentiating the pathogenicity of indica rice blast in South China. Based on the monogenic differentials, cluster and variance analysis method might be applied for the representative isolates selection in the blast resistance evaluation.