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山羊前列腺素内过氧化物合酶2基因的多态性检测及生物信息学分析
引用本文:宋桃伟,罗卫星,蔡惠芬,刘若余,陈志,惠嫣婷. 山羊前列腺素内过氧化物合酶2基因的多态性检测及生物信息学分析[J]. 中国畜牧兽医, 2013, 40(2): 121-125
作者姓名:宋桃伟  罗卫星  蔡惠芬  刘若余  陈志  惠嫣婷
作者单位:贵州大学动物科学学院,高原山地动物遗传育种与繁殖教育部重点实验室,贵州省动物遗传育种与繁殖重点实验室, 贵州省动物遗传育种与繁殖人才基地,贵州贵阳 550025
基金项目:贵州省农业科技攻关项目[黔科合NY字(2012)3006号]
摘    要:本试验以贵州省3大地方山羊品种贵州白山羊、贵州黑山羊、黔北麻羊和2个省外地方品种关中奶山羊和内蒙古绒山羊为研究对象,采用DNA池结合PCR直接测序法对山羊前列腺素内过氧化物合酶2(prostaglandin-endoperoxide synthase 2,PTGS2)基因进行单核苷酸多态性检测。结果表明,在5个山羊品种中共检测到6个SNPs位点:A96G、C20T、A239G、T192C、T164A和G91C,其中,A96G和T164A分别位于外显子7和外显子8中,且均为同义突变,其余4个变异位点位于内含子中。生物信息学分析显示,外显子7和8中的2个SNPs位点均导致了mRNA二级结构的改变。

关 键 词:山羊  PTGS2基因  单核苷酸多态性  生物信息学  
收稿时间:2012-09-03

Polymorphism Detection and Bioinformatics Analysis of Prostaglandin-endoperoxide Synthase 2 Gene in Goat
SONG Tao-wei , LUO Wei-xing , CAI Hui-fen , LIU Ruo-yu , CHEN Zhi , HUI Yan-ting. Polymorphism Detection and Bioinformatics Analysis of Prostaglandin-endoperoxide Synthase 2 Gene in Goat[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(2): 121-125
Authors:SONG Tao-wei    LUO Wei-xing    CAI Hui-fen    LIU Ruo-yu    CHEN Zhi    HUI Yan-ting
Affiliation:Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education; Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, Guizhou Talent Base of Animal Genetics, Breeding and Reproduction, College of Animal Science, Guizhou University, Guiyang 550025, China
Abstract:In this study, Guizhou White goat,Guizhou Black goat and Qianbei Ma goat from Guizhou native goat breeds, and Guanzhong Dairy goat and inner Mongolia cashmere goat from other province were selected for testing subjects, the technology of direct sequencing of PCR products was used to detect the single nucleotide polymorphism of prostaglandin-endoperoxide synthase 2(PTGS2) gene in goats. The consequence showed that six SNPs(A96G,C20T,A239G,T192C,T164A and G91C) were found, among these SNPs, A96G and T164A lay in the exon 7 and exon 8, which were sense mutation and the others lay in intron. Bioinformatics analysis revealed that the two SNPs in the exon 7 and exon 8 lead to the alteration of secondary structure of mRNA.
Keywords:goat  PTGS2 gene  SNP  bioinformatics
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