中国柚类生态分布多样性研究

利用分子标记AFLP技术分析了我国柚类生态分布遗传多样性,根据我国自然形成的西南及长江流域、东南沿海、华南柚产区等3个分布带,其中种群内平均预期杂合度为0.210 4,种群间平均预期杂合度为0.006 8,群体内遗传分化程度Fst为0.031 1,即种群内的遗传多样性所占的比例为96.87%,种群间变异占总变异的3.13%,3个中心产区间基因流Nm为7.79。以西南及长江流域遗传多样性最高,其次为东南沿海,华南柚产区遗传多样性最小。按照内陆性与海洋性两大系统进行柚类遗传多样性分析,所有位点总的预期杂合度为0.224 7,其中种群内平均预期杂合度为0.219 1,种群间平均预期杂合度为0.005 7,群体内遗传分化程度Fst为0.025 1,即种群内的遗传多样性所占的比例为97.51%,种群间变异占总变异的2.5%,2个系统间基因流Nm为9.7。预期杂合度内陆性系统(0.229)高于海洋性系统(0.209)。

A Study on the Ecological Distribution of Pummelo in China by Means of AFLP

The genetic diversity of ecological distribution Pummelo in China was analyzed by using AFLP and the Pummelo varieties were divided according to the 3 main producing areas formed naturally in China.The average heterozygosity within groups was 0.210 4,the heterozygosity between groups was 0.0068,The wright's fixation index(Fst) within groups was 0.031 1.The percentage of genetic diversity within groups was 96.87% compared to 3.13% of the genetic diversity between groups.The gene flow(Nm) between 3 groups was 7.79.The highest genetic diversity value within groups was in the Southwest and Yangtze River valley,followed by Southeastern coastal region,and the smallest value was in the South of China.The genetic diversity of Pummelo was analyzed according to inland and ocean systems.The total heterozygosity of all loci was 0.224 7,in which the heterozygosity within groups was 0.219 1,the heterozygosity between groups was(0.005 7),the wright's fixation index(Fst) within groups was 0.025 1.The percentage of the genetic diversity within groups was 97.51% compared to 2.5% of the genetic diversity between groups.The gene flow(Nm) between 2 systems was 9.7.The heterozygosity of the inland system was 0.229,which was higher than 0.209 of the ocean system.