| 拟南芥抗灰霉病相关基因的克隆与原核表达 |
| |
| 引用本文： | 陈展,郝丛丛,李莉,邢继红,董金皋. 拟南芥抗灰霉病相关基因的克隆与原核表达[J]. 河北农业大学学报, 2010, 33(5) |
| |
| 作者姓名： | 陈展 郝丛丛 李莉 邢继红 董金皋 |
| |
| 作者单位： | 河北农业大学真菌毒素实验室,河北保定,071001;河北农业大学真菌毒素实验室,河北保定,071001;河北农业大学真菌毒素实验室,河北保定,071001;河北农业大学真菌毒素实验室,河北保定,071001;河北农业大学真菌毒素实验室,河北保定,071001 |
| |
| 基金项目： | 河北省自然科学基金项目,河北农业大学9816科技重点项目资助 |
| |
| 摘 要： | 为了从拟南芥中获得抗灰霉病相关基因以及进行基因分析,本试验利用RACE技术获得了该基因的cD-NA全长(1190 bp),并用生物信息学方法和Southern Blotting技术明确了该基因是编码372个氨基酸的转录调节/结合蛋白的AT5G67480基因,以单拷贝形式存在于拟南芥基因组中。编码的蛋白含有BTB/POZ结构域,体外诱导表达的蛋白对灰霉病菌的生长有一定的抑制作用。
|
| 关 键 词： | 拟南芥 灰葡萄孢 RACE 原核表达 |
Cloning and prokaryotic expression of resistant-related gene of Arabidopsis against Botrytis cinerea |
| |
| CHEN Zhan,HAO Cong-cong,LI Li,XING Ji-hong,DONG Jin-gao. Cloning and prokaryotic expression of resistant-related gene of Arabidopsis against Botrytis cinerea[J]. Journal of Agricultural University of Hebei, 2010, 33(5) |
| |
| Authors: | CHEN Zhan HAO Cong-cong LI Li XING Ji-hong DONG Jin-gao |
| |
| Affiliation: | CHEN Zhan,HAO Cong-cong,LI Li,XING Ji-hong,DONG Jin-gao(Mycotoxin Lab.,Agricultural University of Hebei,Baoding 071001,China) |
| |
| Abstract: | Full-length cDNA sequence of resistant-related gene of Arabidopsis against Botrytis cinerea was obtained by running RACE.Result of homology indicated that gene identified as AT5G67480 gene,which encoded binding protein or transcription regulator,containing 372 amino acids.AT5G67480 gene is a single copy in Arabidopsis genomic DNA by Southern Blotting analysis.The protein encodes by AT5G67480 gene contains BTB/POZ domain of TAZ zinc protein family.In most cases,protein containing BTB/POZ domain is related to... |
| |
| Keywords: | RACE |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
