| 花生幼苗多胺氧化酶的分离提取 |
| |
| 引用本文： | 林淑华,李均,贺莹,何生根. 花生幼苗多胺氧化酶的分离提取[J]. 仲恺农业技术学院学报, 2007, 20(2): 27-30 |
| |
| 作者姓名： | 林淑华 李均 贺莹 何生根 |
| |
| 作者单位： | 仲恺农业技术学院,生命科学学院,广东,广州,510225 |
| |
| 基金项目： | 广东省自然科学基金;广东省教育厅优秀人才培养基金;广东省质监局农业标准化专项;仲恺农业技术学院学生创新基金 |
| |
| 摘 要： | 对花生(Arachis hypogaeaL.)幼苗多胺氧化酶(Polyamine oxidase,PAO)的分离提取方法进行了研究,内容涉及PAO活性在花生幼苗各器官中的分布以及在不同萌发时期的变化、酶提取液组分对PAO提取效果的影响.结果表明,在黑暗条件下萌发的花生幼苗,其胚根和胚轴的PAO活性分别在萌发后的第4 d和第5 d出现一个峰值;含1%PVP和0.5 M NaCl的磷酸缓冲液(0.1 mol/L,pH7.0)用作花生PAO分离提取液可取得较好效果.
|
| 关 键 词： | 花生Arachis hypogaea L. 多胺氧化酶 分离提取 |
| 文章编号： | 1006-0774（2007）02-0027-04 |
| 收稿时间： | 2006-11-25 |
| 修稿时间： | 2006-11-25 |
Extraction of polyamine oxidase from peanut (Arachis hypogaea L. ) seedlings |
| |
| LIN Shu-hua,LI Jun,HE Yin,HE Sheng-gen. Extraction of polyamine oxidase from peanut (Arachis hypogaea L. ) seedlings[J]. Journal of Zhongkai Agrotechnical College, 2007, 20(2): 27-30 |
| |
| Authors: | LIN Shu-hua LI Jun HE Yin HE Sheng-gen |
| |
| Affiliation: | College of Life Sciences, Zhongkai University of Agriculture and Technology, Guangzhou 510225, China |
| |
| Abstract: | A series of experiments concerning the extraction of polyamine oxidase from peanut(Arachis hypogaea L.) were conducted,including distribution of PAO activity in peanut young seedlings and its change during the germination period.It was found that the highest PAO activities in embryonic axes and radicles of the peanut seedlings occurred in day 4 and day 5 respectively after germination in dark.Additionally,the extraction medium of peanut PAO was 0.1 mol/L phosphate-buffered saline(pH7.0),containing 0.5 mol/L NaCl and 1%(g/V) polyvinylpyrrolidone. |
| |
| Keywords: | Arachis hypogaea L. polyamine oxidase extraction |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
