| 植物游离小孢子及其培养所获得的组织在遗传转化中的应用 |
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| 引用本文： | 常凯军,谭德冠,张家明. 植物游离小孢子及其培养所获得的组织在遗传转化中的应用[J]. 中国农学通报, 2009, 25(15): 16-1 |
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| 作者姓名： | 常凯军 谭德冠 张家明 |
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| 作者单位： | 1. 中国热带农业科学院热带生物技术研究所,农业部热带作物生物技术重点开放实验室,海口,571101
2. Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences,Key laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Haikou 571101 |
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| 基金项目： | 国家自然科学基金，中国热带农业科学院中央级公益性科研院所基本科研业务费专项基金 |
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| 摘 要： | 植物游离小孢子及其培养所获得的组织是植物基因工程中优良的受体，它们具有单倍性和较高胚胎发生能力的特性。因此，以它们为受体构建遗传转化体系可以快速获得纯合的转基因植株。通过综述植物游离小孢子及其培养所获得的胚状体、愈伤组织作为转化受体在根癌农杆菌介导法、基因枪轰击法、激光微孔穿刺法、显微注射法、电激法、PEG介导法等转化技术中的应用研究进展，对目前游离小孢子作为转化受体存在的问题及发展前景进行了探讨。
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| 关 键 词： | 游离小孢子 游离小孢子培养来源的胚状体 遗传转化 根癌农杆菌介导法 基因枪轰击法 |
| 收稿时间： | 2009-03-05 |
| 修稿时间： | 2009-04-02 |
Applications of Plant Isolated Microspores and Microspore-derived Tissues in Genetic Transformation |
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| Chang Kaijun,Tan Deguan,Li Dingqin,Zheng Peng,Zhang,Jiaming. Applications of Plant Isolated Microspores and Microspore-derived Tissues in Genetic Transformation[J]. Chinese Agricultural Science Bulletin, 2009, 25(15): 16-1 |
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| Authors: | Chang Kaijun Tan Deguan Li Dingqin Zheng Peng Zhang Jiaming |
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| Affiliation: | (Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Key laboratory of Tropical Crop Biotechnology, Ministry of Agriculture, Haikou 571101 ) |
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| Abstract: | Based on their characteristics of haploid and high embryogenetic capacity, plant isolated micro-spores and microspore-derived tissues are the good acceptors in plant genetic engineering. Therefore, the ho-mozygous transgenic plants could be obtained more quickly using them as acceptors to construct genetic trans-formation system. The applications of plant isolated microspores, microspore-derived embryos and micro-spore-derived calli as acceptors in several transformational technology including Agrobacterium co-cultiva-tion, particle bombardment, laser microbeam puncture, microinjection, electroporation, polyethylene glycol (PEG)-mediated were reviewed. Furthermore, problems and prospects of microspores as transformation accep-tors were discussed in this review. |
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| Keywords: | isolated microspore microspore-derived embryo genetic transformation Agrobacterium media-tion particle bombardment |
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