| Abstract: | [Objective] Through inductive culture, emedin and ehrysophanol contents in cassia seed were increased. [Method]MS culture medium was used to bourgcon seedlings of cassia seed and to induce cotyledon callus of cassia seed while HPLC method was adopted to determine emodin and chry-sophanol contents. [Result] Emedin and chrysophanol contents in cotyledon callus were 0.099% and 0.312%, respectively, while they were 0.029% and 0.190% respectively in cassia seed. [Conclusion]The method of inducing cotyledon callus was helpful for increasing emedin content in cassia seed. |
