地肤内生细菌对番茄灰霉病菌的拮抗作用

从植物内生细菌中筛选拮抗活性菌株和寻找新的农用活性代谢产物,可为植物病害防治提供新的资源。本研究从地肤中分离筛选得到5株对番茄灰霉病菌具有抑制作用的内生细菌,其中D-29菌株对在离体番茄上对灰霉病菌具有强烈的抑制作用。D-29发酵液、细菌悬浮液、无菌滤液在离体红番茄上对灰霉病抑制率分别为64.61%、61.50%、44.10%。发酵液在离体青番茄上对灰霉病菌的抑制率4d时为100%、6d时为67.11%。D-29菌株可使灰霉病菌部分菌丝顶端生长畸形,胞内物质外泄,产孢梗畸形。用不同浓度的D-29无菌滤液处理病菌孢子,100%D-29无菌滤液抑制效果最明显,抑制率高达80%。

Antagonism of Endophyte Bacteria from Kochia scoparia on Botrytis cinerea

The aim of the study is to screen anti-microorganism endophytic bacteria for plant disease control.Five strains from the Kochia scoparia exhibited extensive antagonistic activities against Botrytis cinerea.D-29 strain showed strong antagonistic activity against Botrytis cinerea on tomato in vitro.The fermentation solution,suspension of bacteria and sterile filtrate could reduce the expension of leisons on red tomato in vitro and inhibitory rate reached 64.61%,61.50% and 44.10% respectively.Fermentation solu...