| 野桑蚕多酚氧化酶分离纯化的试验初报 |
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| 引用本文： | 张永亮,朱勇. 野桑蚕多酚氧化酶分离纯化的试验初报[J]. 蚕业科学, 2009, 35(1) |
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| 作者姓名： | 张永亮 朱勇 |
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| 作者单位： | 周口师范学院生命科学系,河南,周口,466001;西南大学生物技术学院,重庆,400715 |
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| 基金项目： | 教育部高等学校博士学科点专项科研基金，重庆市教委科学基金 |
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| 摘 要： | 多酚氧化酶是昆虫初生新表皮硬化过程中的关键性酶。为有利于研究野桑蚕(Bombyxmandarina)多酚氧化酶的功能与性质,探讨了野桑蚕多酚氧化酶的分离纯化方法。以野桑蚕5龄幼虫为材料,用磷酸盐缓冲液浸提多酚氧化酶粗提液,再经40%饱和度硫酸铵沉淀、透析及Sephadex G-100凝胶过滤进一步分离纯化,得到了部分纯化的多酚氧化酶,其酶纯化倍数为57.14倍±0.19倍,酶得率为10.17%±0.18%,酶比活力为(10 166.67±66.16)U/mg。采用该试验方法可有效分离纯化野桑蚕多酚氧化酶。
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| 关 键 词： | 野桑蚕 多酚氧化酶 分离 纯化 |
A Preliminary Report on Extraction and Purification of Polyphenol Oxidase From Bombyx mandarina |
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| ZHANG Yong-Liang,ZHU Yong. A Preliminary Report on Extraction and Purification of Polyphenol Oxidase From Bombyx mandarina[J]. Acta Sericologica Sinica, 2009, 35(1) |
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| Authors: | ZHANG Yong-Liang ZHU Yong |
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| Affiliation: | ZHANG Yong-Liang1 ZHU Yong2(1Department of Life Sciences,Zhoukou Normal University,Zhoukou Henan 466001,China,2School of Biotechnolgy,Southwest University,Chongqing 400715,China) |
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| Abstract: | Polyphenol oxidase is a key enzyme involved in the sclerotization of new cuticular layer of insect.In order to study the function and characters of polyphenol oxidase from the wild silkworm Bombyx mandarina,isolation and purification methods of polyphenol oxidase from Bombyx mandarina were approached.Crude polyphenol oxidase was extracted from the 5th instar larvae with sodium phosphate extraction buffer.Partially purified polyphenol oxidase was obtained after precipitation with 40% saturated(NH4)2SO4,dialy... |
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| Keywords: | Bombyx mandarina Polyphenol oxidase Isolation Purification |
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