| 培养基组成对桔梗组织培养的影响 |
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| 引用本文： | 吴京姬,吴基日,严一字,吴松权,李美善. 培养基组成对桔梗组织培养的影响[J]. 延边大学农学学报, 2006, 28(1): 18-23 |
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| 作者姓名： | 吴京姬 吴基日 严一字 吴松权 李美善 |
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| 作者单位： | 延边大学农学院,吉林,龙井,133400 |
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| 摘 要： | 以MS和N6为基本培养基，配置不同激素种类和浓度，以桔梗的叶片和茎段为外植体，比较培养基组成对桔梗组织培养的影响．结果表明：基本培养基的组成和2，4-D浓度对桔梗组织培养的影响较大，以N6为基本培养基的培养效果明显好于MS；低浓度2，4-D的培养效果明显好于高浓度的2，4-D；以叶片为外植体的培养效果明显好于茎段．因此，在桔梗组织培养中以N6为基本培养基，叶片为外植体，而生长素则以NAA或IAA来代替2，4-D，能够提高桔梗组织培养的效率．
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| 关 键 词： | 桔梗 组织培养 基本培养基 2,4-D浓度 |
| 文章编号： | 1004-7999(2006)01-0018-06 |
| 收稿时间： | 2005-11-25 |
| 修稿时间： | 2005-11-25 |
Effects of media compositions on tissue culture of Platycodon grandiflorum(Jacp.)A.DC. |
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| WU Jing-ji,WU Ji-ri,YAN Yi-zi,WU Song-quan,LI Mei-shan. Effects of media compositions on tissue culture of Platycodon grandiflorum(Jacp.)A.DC.[J]. Journal of Agricultural Science Yanbian University, 2006, 28(1): 18-23 |
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| Authors: | WU Jing-ji WU Ji-ri YAN Yi-zi WU Song-quan LI Mei-shan |
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| Affiliation: | Agricultural College of Yanbian University, Longjing Jilin 133400, China |
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| Abstract: | The effects of media compositions on tissue culture of Platycodon grandiflorum(Jacp.)A.DC were compared,using the basic MS and N_6 medium of different sorts and concentrations of phytohormone in the explants of leaves and stems.The results showed that the compositions of media and concentrations of 2,4-D had distinct effects on the tissue culture;N_6 tissue culture had better effect than MS tissue culture;lower concentration of 2,4-D had better effect than higher concentration,and the explant of leaves had better effect than that of stem.Therefore,N_6 tissue culture with NAA or IAA instead of 2,4-D in the explants of leaves could increase the efficiency of tissue culture of Platycodon grandiflorum(Jacp.)A.DC.. |
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| Keywords: | Platycodon grandiflorum(Jacp.)A.DC. tissue culture basic medium (2 4-D concentration) |
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