| 狂犬病病毒CVS-11株的培养及其效价测定 |
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| 引用本文： | 张传明,冷雪梅,蒋雯雯,杨敏. 狂犬病病毒CVS-11株的培养及其效价测定[J]. 现代畜牧兽医, 2014, 0(7): 42-44 |
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| 作者姓名： | 张传明 冷雪梅 蒋雯雯 杨敏 |
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| 作者单位： | 常州同泰生物药业科技有限公司,江苏常州213136 |
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| 摘 要： | 本研究通过神经瘤细胞（NA）培养CVS-11株狂犬病病毒,并测定不同批次的病毒毒价,为优化狂犬病疫苗的生产工艺提供数据支持,具有实际意义。病毒培养和检测结果表明,第1次和第2次收获的病毒液病毒含量较高,在107.0FFU/0.1 mL以上,毒力为106.4～107.0LD50/0.03 mL;而第3次收获的病毒含量则较低,只有106.0FFU/0.1 mL左右,毒力为105.0～105.3LD50/0.03 mL。第1次和第2次收获的病毒液可用于制备狂犬疫苗,第3次收获的病毒液因毒价较低不适于制备狂犬疫苗。
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| 关 键 词： | 神经瘤细胞 狂犬病病毒CVS-11株 病毒含量 病毒毒力 |
Culture and Potency Titration of Rabies Virus Strain CVS-11 |
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| Zhang Chuanming;Leng Xuemei;Jiang Wenwen;Yang Min. Culture and Potency Titration of Rabies Virus Strain CVS-11[J]. Modern JOurnal of Animal Husbandry and Veterinary Medicine, 2014, 0(7): 42-44 |
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| Authors: | Zhang Chuanming Leng Xuemei Jiang Wenwen Yang Min |
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| Affiliation: | Zhang Chuanming;Leng Xuemei;Jiang Wenwen;Yang Min;(Changzhou Tongtai Bio-pharmaceutics and Technology Enterprise,Ltd.; Jiangsu Changzhou 213136) |
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| Abstract: | Through cultivating Rabies virus strain CVS-11 on Neuroma cells, and testing the ti-ter and potency of different batches of harvested fluid, to get the experiment data for Rabies vaccine manufacture and process optimization. The first and second harvesting virus titers were higher than 107.0 FFU/0.1 mL, virus virulence between 106.4 and 107.0 LD50/0.03 mL, and can be used of manufacture Rabies vaccine. The third harvesting virus titer was about 106.0 FFU/0.1 mL, virus virulence was between 105.0 and 105.3 LD50/0.03 mL, and was not useful for Rabies vaccine manufacture. |
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| Keywords: | Neuroma cell Rabies virus strain CVS-11 Virus titer Virus virulence |
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