枇杷花药培养诱导愈伤组织的研究

为了寻找枇杷花药愈伤组织诱导的最佳条件及配方。以枇杷花药为试验材料,采用完全组合设计研究了温度、蔗糖浓度、激素对花药愈伤组织诱导的影响。低温处理对愈伤组织的形成没有多大影响,随着低温时间的延长,愈伤组织诱导率低于对照;30 g/L蔗糖诱导花药愈伤组织的效果较好,诱导率为62.5%;在附加1 mg/L 2,4-D和2 mg/L 6-BA的MS培养基上,诱导率最高,为90.00%;MS+0.25mg/L6-BA+1 mg/LNAA最有利于愈伤组织的增殖。高浓度的蔗糖不利于愈伤组织的形成。一定浓度的2,4-D和6-BA对枇杷花药愈伤组织的诱导有较大的影响,且2,4-D起着主导作用。

Study an Callus Induction in Loquat Anther Culture

The purpose was to find the optimum conditions and formula of inducing loquat anther callus.With loquat anther as tested material,the influences of temperature,sucrose concentration and hormone on inducing loquat anther callus were studied by complete combination design.Low temperature treatment made little influences on callus formation.Compared with CK,the callus induction was lower along with low-temperature time prolonging.The effect of inducing anther callus with 30 g/L sucrose was better,with the induction rate of 62.5%.The induction rate of MS medium added with 1 mg/L 2,4-D and 2 mg/L 6-BA was highest,being 90.00%.The medium of MS 0.25 mg/L6-BA 1 mg/LNAA was most favorable to callus proliferation.The sucrose with high concentration was not favorable to callus formation.2,4-D and 6-BA with certain concentration had greater influences on loquat anther callus and 2,4-D played leading role.