| 大蒜茎尖脱毒及组织培养研究 |
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| 引用本文： | 海燕,康明辉,何宁,郭景战,张丹,夏祖灵.大蒜茎尖脱毒及组织培养研究[J].河南农业科学,2006(11):97-98. |
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| 作者姓名： | 海燕 康明辉 何宁 郭景战 张丹 夏祖灵 |
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| 作者单位： | 1. 河南省农作物新品种重点实验室,河南,郑州,450002
2. 杞县农业局,河南,杞县,475200 |
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| 基金项目： | 科技部农业科技成果转化基金 |
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| 摘 要： | 采用茎尖分生组织培养技术,获得了大蒜无病毒试管苗。通过激素配比试验,筛选出最佳的培养基组成,进行脱病毒苗的快速繁殖。结果表明:诱导茎尖出芽的最适培养基为MS 6 BA 1.5mg/L NAA 1.0mg/L,诱导生根的最适培养基为MS IAA 2.0mg/L.
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| 关 键 词： | 大蒜 茎尖培养 脱病毒 |
| 文章编号： | 1004-3268(2006)11-0097-02 |
| 收稿时间： | 2006-04-11 |
| 修稿时间： | 2006年4月11日 |
Study on Virus-free Technology of Shoot-tip and Tissue Culture in Allium sativum |
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| HAI Yan,KANG Ming-hui,HE Ning,GUO Jing-zhan,ZHANG Dan,XIA Zu-ling.Study on Virus-free Technology of Shoot-tip and Tissue Culture in Allium sativum[J].Journal of Henan Agricultural Sciences,2006(11):97-98. |
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| Authors: | HAI Yan KANG Ming-hui HE Ning GUO Jing-zhan ZHANG Dan XIA Zu-ling |
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| Institution: | 1. Henan Key l.aboratory for Crop Improvement, Zhengzhou 450002, China; 2. Qixian Agricultural Bureau of Henan, Qixian 475200, China |
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| Abstract: | Virus-free materials of Allium sativum L.were obtained from in vitro shoot-tip tissue culture.A series of optimization experiments for concentration of hormones were investigated.The results showed that the induction frequency of stem-tips was the best when the medium was MS containing 6BA 1.5mg/L and NAA 1.0mg/L and the ratio of the induction of roots was the most when the medium was MS supplemented with IAA 2.0mg/L. |
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| Keywords: | Garlic Shoot-tip culture Virus-free |
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