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| H9亚型禽流感病毒血凝素蛋白在原核系统中的表达 | |
| 引用本文： | 石锐,包红梅,姜永萍,乔传玲,陈化兰,王秀荣,陈维多. H9亚型禽流感病毒血凝素蛋白在原核系统中的表达[J]. 东北农业大学学报, 2011, 42(12): 86-90 |
| 作者姓名： | 石锐 包红梅 姜永萍 乔传玲 陈化兰 王秀荣 陈维多 |
| 作者单位： | 1. 黑龙江民族职业学院,哈尔滨,150081 2. 中国农业科学院哈尔滨兽医研究所农业部动物流感重点开放实验室及兽医生物技术国家重点实验室,哈尔滨,150001 3. 东北农业大学生命科学学院,哈尔滨,150030 |
| 摘 要： | 应用带有His6尾的pET原核表达系统对禽流感病毒血凝素蛋白进行表达,将禽流感A/Turkey/Wisconsin/66(HgN2)的HA基因克隆至原核表达载体pET-30a中,经酶切、PCR扩增和测序分析确证其插入,并且阅读框正确,获得重组质粒pET30a-H9.将此重组质粒转化到宿主菌BL21中,用诱导剂异丙基硫代... |
| 关 键 词： | 禽流感 H9 原核表达 |
Expression of HA protein of H9 subtype avian influenza virus in prokaryotic system |
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| SHI Rui,BAO Hongmei,JIANG Yongping,QIAO Chuanling,CHEN Hualan,WANG Xiurong,CHEN Weiduo. Expression of HA protein of H9 subtype avian influenza virus in prokaryotic system[J]. Journal of Northeast Agricultural University, 2011, 42(12): 86-90 | |
| Authors: | SHI Rui BAO Hongmei JIANG Yongping QIAO Chuanling CHEN Hualan WANG Xiurong CHEN Weiduo |
| Affiliation: | 1.Heilonjiang Vocational College for Nationalities,Harbin 150081,China;2.Animal Influenza Laboratory of Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology of Harbin Veterinary Research Institute of CAAS,Harbin 150001,China;3.School of Life Sciences,Northest Agriculture University,Harbin 150030,China) |
| Abstract: | The HA protein of avian influenza virus(AIV) was expressed by pET expression systems in E.coli.The Hemagglutinin(HA)gene of A/Turkey/Wisconsin/66(H9N2)strain has been cloned into a prokaryotic expression plasmid pET30a with 6-His tag.That recombinant vector pET30a-H9 was transformed into E.coli.BL21,which was induction with IPTG,and product was collected.The expressed protein was identified with SDS-PAGE and Western Blotting.When the OD600 value of the bacteria containing pET30a-H9 was 1.0,the recombinant protein was highly expressed in E.coli BL21 at four hours later induced by 0.3 mmol·L-1 IPTG in the form of inclusion bodies.And the protein is about 64 Ku in size,the rate of the expressed protein in the induced bacteria protein was about 35%.Western-bolt analysis with AIV antibodies against H9 subtype showed the recombinant protein was distinguished clearly and specially. |
| Keywords: | AIV H9 prokaryotic expression |
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