不同培养基及贮藏条件对山梅花属植物花粉生活力的影响

以欧洲山梅花(Philadelphus coronaries Aureus)、庆典山梅花(Philadelphus‘Galahad’)、东北山梅花(P.schrenkii Rupr.)、京山梅花(P.pekinensis Rupr.)4个山梅花植物为试材,采用正交试验方法对影响花粉生活力的3个因素(蔗糖、硼酸和培养温度...

Effects of Different Culture Media and Storage Condition on Pollen Viability of Philadelphus

Philadelphus coronaries Aureus,Philadelphus ‘Galahad’,P.schrenkii Rupr.and P.pekinensis Rupr.were used as experimental materials to study the effects of culture media(sucrose and boric acid) and storage temperature on pollen viability.Orthogonal design of three factors at three levels(sucrose content,boric acid content and culture temperature) was applied to optimize the conditions of pollen germination.Results show that there are differences in pollen germination rate between different species.The germination rates of P.coronaries and P.‘Galahad’ were lower than those of P.schrenkii.and P.pekinensis.Boric acid content showed the most obvious effect on pollen germination rate of P.schrenkii,followed by sucrose content and culture temperature.However,sucrose exhibited the most obvious effect on pollen germination rate of the other three species,followed by boric acid and culture temperature.The culture temperature ranging from 20 to 30 degrees C had no significant effect on pollen germination rate.The suitable conditions for pollen germination of the four species were obtained according to the results of orthogonal design.A single factor test was used to optimize the storage conditions of pollen germination at three storage temperatures(-23,3,and 20 degrees C).The result shows that the best storage temperature for the four species of Philadelphus is 3 degrees C.