| 甘草次酸在Caco-2细胞模型中的转运机制 |
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| 引用本文： | 王筱萌,祖元刚,王微,付玉杰,张琳. 甘草次酸在Caco-2细胞模型中的转运机制[J]. 东北林业大学学报, 2011, 39(12): 100-103 |
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| 作者姓名： | 王筱萌 祖元刚 王微 付玉杰 张琳 |
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| 作者单位： | 森林植物生态学教育部重点实验室(东北林业大学) 哈尔滨 150040 |
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| 摘 要： | 采用液相-质谱检测甘草次酸在HBSS磷酸盐缓冲液中的转运量,结果表明:甘草次酸的转运量受时间、浓度以及p-糖蛋白抑制剂维拉帕米和汉防己甲素的影响,表观渗透系数P(appBL-AP)为(17.1±4.2) ×10-6～(54.3±6.3) ×10-6cm·s-1、P(appAP-BL)为(15.6-±3.4) ×10-6...
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| 关 键 词： | 甘草次酸 Caco-2细胞模型 双向转运 P-糖蛋白 抑制剂 |
Transport Mechanism of Glycyrrhetinic Acid in Human Intestinal Epithelial Caco-2 Cells |
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| Wang Xiaomeng,Zu Yuangang,Wang Wei,Fu Yuiie,Zhang Lin. Transport Mechanism of Glycyrrhetinic Acid in Human Intestinal Epithelial Caco-2 Cells[J]. Journal of Northeast Forestry University, 2011, 39(12): 100-103 |
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| Authors: | Wang Xiaomeng Zu Yuangang Wang Wei Fu Yuiie Zhang Lin |
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| Affiliation: | (Key Laboratory of Forest Plant Ecology(Northeast Forestry University),Ministry of Education,Harbin 150040,P.R.China) |
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| Abstract: | An experiment was conducted to determine the transferred amount of glycyrrhetinic acid in phosphate buffered solution by HPLC-MS/MS,in order to study the transport mechanism of glycyrrhetinic acid with Caco-2 cell monolayers.Results showed that the transferred amount of glycyrrhetinic acid was affected by culture time,drug concentration and P-glycoprotein inhibitor verapamil and Tetrandrine.The apparent permeability coefficients of glycyrrhetinic acid were(17.1±4.2)×10-6-(54.3±6.3)×10-6cm·s-1 for PappBL-AP,and(15.6±3.4)×10-6cm·s-1 for PappAP-BL,and the bidirectional transport characteristics were different.It indicated that both active transport and passive diffusion contributed to the absorption of glycyrrhetinic acid in Caco-2 cell monolayers,but the passive diffusion was the dominant process.The P-glycoprotein may play an important role in the efflux of glycyrrhetinic acid. |
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| Keywords: | Glycyrrhetinic acid Caco-2 cell model Bidirectional transport P-glycoprotein P-glycoprotein inhibitors |
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