| 一株蚕蛹壳几丁质降解菌的分离与初步鉴定 |
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| 引用本文： | 赵祥杰,邝哲师,叶明强,罗国庆,肖更生.一株蚕蛹壳几丁质降解菌的分离与初步鉴定[J].蚕业科学,2009,35(2). |
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| 作者姓名： | 赵祥杰 邝哲师 叶明强 罗国庆 肖更生 |
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| 作者单位： | 广东省农业科学院农业生物技术研究所,广东省农业科学院蚕业与农产品加工研究所,广州,510610 |
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| 基金项目： | 广东省农业攻关计划项目，粤港关键领域重点突破招标项目 |
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| 摘 要： | 利用几丁质降解菌可有效提高蚕蛹壳在综合利用加工过程中的降解率。从蚕蛹储藏车间及周围的土壤中取样,经过富集培养和蚕蛹壳胶体几丁质驯化,分离并筛选出能产高活性几丁质酶的细菌菌株G-254。根据形态学观察、生理生化试验以及16S rDNA序列测定与系统发育分析,初步将该菌株归属为嗜麦芽寡养单胞菌(Stenotrophomonas maltophilia)。
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| 关 键 词： | 蚕蛹壳 几丁质降解菌 分离 鉴定 嗜麦芽寡养单胞菌 G-254菌株 |
Isolation and Preliminary Identification of a Bacterial Strain That Degrades Chitin of Silkworm Pupal Cases |
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| ZHAO Xiang-Jie,KUANG Zhe-Shi,YE Ming-Qiang,LUO Guo-Qing,XIAO Geng-Sheng.Isolation and Preliminary Identification of a Bacterial Strain That Degrades Chitin of Silkworm Pupal Cases[J].Acta Sericologica Sinica,2009,35(2). |
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| Authors: | ZHAO Xiang-Jie KUANG Zhe-Shi YE Ming-Qiang LUO Guo-Qing XIAO Geng-Sheng |
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| Institution: | ZHAO Xiang-Jie KUANG Zhe-Shi YE Ming-Qiang LUO Guo-Qing XIAO Geng-Sheng (The Agricultural Biotechnology Research Institute,Guangdong Academy of Agricultural Sciences,The Sericulture & Farm Produce Processing Research Institute,Guangzhou 510610,China) |
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| Abstract: | In order to increase the degradation rate in the comprehensive utilization of silkworm pupal cases,an excellent chitinolytic bacterial strain was isolated from soil and water samples taken from the silkworm pupa storage house and its surrounding area.After enrichment culture and acclimation with silkworm pupal case chitin colloid,a bacterial strain with high chitinase activity was obtained and named as G-254.Based on morphological observation,physio-biochemical analysis,16S rDNA sequencing and phylogenetic ... |
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| Keywords: | Silkworm pupal case Chitin degradation bacterium Isolation Identification Stenotrophomonas maltophilia Strain G-254 |
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