中华鲟GH蛋白二级结构和B细胞抗原表位的预测

笔者采用RT—PCR技术从中华鲟脑垂体中克隆出了生长激素（GH）基因，该文根据已获得的GH氨基酸序列一级结构，运用计算机技术和分子生物学软件对中华鲟GH蛋白二级结构和B细胞表位进行分析和预测，为中华鲟GH蛋白单克隆抗体的制备提供依据，为探讨中华鲟分子调控机理提供参考。

Prediction of Secondary Structure and B Cell Epitope of GH Protein from Acipenser sinensis

[Objective] The aim was to predict the secondary structure and B cell epitope of growth hormone (GH) protein from Acipenser sinensis. [Method] With the amino acid sequence of GH protein from A.sinensis as the base, the secondary structure of GH protein from A.sinensis was predicted by the method of Garnier-Robson, Chou-Fasman and Karplus-Schulz, and its cell epitope was predicted by the method of Kyte-Doolittle, Emini and Jameson-Wolf. [Result] The sections of 18-23, 55-56, 67-73, 83-86, 112-114, 151-157 and 209-211 in the N terminal of GH protein molecule had softer structure and these sections could sway or fold to produce more complex tertiary structure. The sections of 19-23, 51-71, 84-95, 128-139, 164-176 and 189-196 in the N terminal of GH protein could be the epitope of B cell and there were flexible regions in these sections or near these sections of GH protein molecule. So the dominant regions could be in these sections or near these sections. [Conclusion] The research provided the basis for the preparation of monoclonal antibody of GH protein from A.sinensis and provided the reference for the discussion for the molecular regulation mechanism of A.sinensis.