3种猫疱疹病毒Ⅰ型检测方法的比较

比较传统PCR、荧光定量PCR以及胶体金速测卡对于猫疱疹病毒Ⅰ型（FHV-1）检测的敏感性及准确性。设计引物，优化反应条件并扩增目的片段，定向插入pMD-19T克隆载体，最后用建立的2种PCR检测方法及猫疱疹病毒1型快速检测卡对21份临床疑似猫疱疹病毒感染的分泌物进行检测。荧光定量PCR和传统PCR检测的最低拷贝数分别为20拷贝和207拷贝；荧光定量PCR、传统PCR以及胶体金快速检测卡的检出率分别为71%、57%和29%。传统PCR及荧光定量PCR检测方法均具有良好的特异性，其中荧光定量PCR特异性显著，相比猫疱疹病毒1型快速检测卡敏感性好，准确率更高。

Comparison of detection on feline herpesvirus-1 by three methods

This study compared the sensitivity and accuracy of detection on feline herpesvirus-1 by using three methods of traditional PCR, Real-time?PCR and colloidal gold detection card. Two methods of traditional ?PCR and Real-time PCR based on Taq Man probe technology were established to detect feline herpesvirus-1 in?our lab. The minimum of copies detected by fluorogenic quantitative PCR and traditional PCR were 20 and 207, respectively. The two well?established?methods?in?our?lab?and?the?rapid?detection?card?of Feline herpesvirus-1 were used to detect 21 secretion?suspected?Feline?herpesvirus?infection?clinically. It?showed that the detection rates of Real- time PC, traditional?PCR?and?quick?test?card?were?71%,57%?and?29%, respectively. The result indicated that compared with the rapid detection card of cat herpesvirus type 1, the detection method of traditional PCR and Real- time PCR is?more?sensitive?and?accurate, especially?Real-time?PCR which can provide more accurate ?results for clinical diagnosis.