体重和剂量对链脲佐菌素致糖尿病小鼠模型影响的研究

为了研究体重与剂量对链脲佐菌素致糖尿病小鼠模型的影响,采用不同体重范围的雄性小鼠,对其腹腔注射不同剂量的链脲佐菌素,剂量范围为:100～300mg/(kg·bw),分5次注射造模。在不同时间测量试验鼠的血糖值、死亡率及成模率,观察小鼠的糖尿病发展病程,确定链脲佐菌素最佳用药剂量。结果表明,当给雄性小鼠腹腔注射200mg/(kg·bw)的链脲佐菌素后,造模成功率最高为85%,小鼠表现出典型的糖尿病症状,且成模饲养1周后血糖、体重变化不大,为制备糖尿病小鼠模型的最佳剂量;同等剂量的情况下,低体重小鼠比高体重小鼠的成模率高、死亡率低。

Studies on the Weight and Dose Affecting the Mice Diabetic Model Induced by Streptozocin

In order to study how the weight and dose affect the diabetic model induced by streptozocin, male mice of different weights were intraperitoneal injected into different doses of streptozocin by 5 times, the range of which was from 100 to 300 mg/（kg.bw）. The course of disease, blood glucose, the death rate and the successful diabetic model were recorded to make sure the best dose of making diabetic models for streptozocin. The results showed that the highest rate of making diabetic model was 85% when the male mice were injected into 200 mg/（kg.bw） streptozocin. In that case, those mice showed typical diabetic disorder, and the weight and blood glucose changed little. Therefore, the best dose of making diabetic mice models for streptozotocin was 200 mg/（kg. bw） which was injected by 5 times. By the way, the rate of making successful diabetic models with lower weight of male mice was higher than with higher weight of male mice, and it also had lower death rate.