| 甜瓜SRAP-PCR体系的建立与优化 |
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| 引用本文： | 刘龙洲,朱为民,陈幼源,张永平. 甜瓜SRAP-PCR体系的建立与优化[J]. 分子植物育种, 2009, 7(5): 1045-1047. DOI: 10.3969/mpb.007.001045 |
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| 作者姓名： | 刘龙洲 朱为民 陈幼源 张永平 |
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| 作者单位： | 上海市农业科学院园艺研究所,上海市设施园艺技术重点实验室,上海,201106 |
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| 基金项目： | 农业部都市农业(南方)重点开放实验室开放基金，上海市自然科学基金，上海农业科学院青年基金共同资助 |
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| 摘 要： | 相关序列扩增多态性（sequence—related amplified polymorphism，SRAP）是基于基因的内含子和外显子区域进行检测的新型分子标记技术，具有多态性高、重复性好、便于克隆目标片段等特点，鉴于甜瓜（Cucumis melo L．）中还鲜有SRAP-PCR的相关研究报道。本研究利用L16（4^5）正交设计对甜瓜SRAP-PCR反应体系进行了优化。结果确定了10μLSRAP-PCR的最优反应体系：200ng/μL模板DNA2.5μL、10μmol／L引物0．3μL、5U／μL Toq DNA聚合酶0．6μ20mmol／L Mg^2＋ 1μL和2mmol／L dNTP 2μL。这一优化的反应体系为今后利用SRAP-PCR技术对甜瓜进行相关研究提供了帮助。
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| 关 键 词： | 甜瓜 SRAP-PCR 反应体系 优化 |
Establishment and Optimization for SRAP-PCR System of Muskmelon |
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| Liu Longzhou Zhu Weiming Chen Youyuan Zhang Yongping Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai Key Lab of Protected Horticultural Technology,Shanghai. Establishment and Optimization for SRAP-PCR System of Muskmelon[J]. Molecular Plant Breeding, 2009, 7(5): 1045-1047. DOI: 10.3969/mpb.007.001045 |
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| Authors: | Liu Longzhou Zhu Weiming Chen Youyuan Zhang Yongping Horticultural Research Institute Shanghai Academy of Agricultural Sciences Shanghai Key Lab of Protected Horticultural Technology Shanghai |
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| Affiliation: | Liu Longzhou Zhu Weiming Chen Youyuan Zhang Yongping Horticultural Research Institute,Shanghai Academy of Agricultural Sciences(SAAS),Shanghai Key Lab of Protected Horticultural Technology,Shanghai,201106 |
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| Abstract: | Sequence-related amplified polymorphism(SRAP) is a kind of novel molecular marker technique based on detecting the regions of intron and extron,which owns the excellent characters such as high polymorphism,good reproduciblity,and also facilitates to clone the target gene.Whereas application for this technique on muskmelon was quite few reported yet,we attempted to establish and optimize SRAP-PCR amplification system of muskmekon by employing L16(45) orthogonal design with five factors and four levels,i.e.te... |
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| Keywords: | SRAP-PCR |
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