| 耳鲍血淋巴抗菌活性的研究 |
| |
| 引用本文： | 张英霞,满初日嘎.耳鲍血淋巴抗菌活性的研究[J].水产科学,2009,28(5). |
| |
| 作者姓名： | 张英霞 满初日嘎 |
| |
| 作者单位： | 1. 海南大学,海洋学院,热带生物资源教育部重点实验室,海南省热带水生生物技术重点实验室,海南,海口,570228
2. 海南大学,农学院,海南,海口,570228 |
| |
| 基金项目： | 海南省教育厅高等学校科研项目，海南省热带水生生物技术重点实验室开放基金 |
| |
| 摘 要： | 用溶藻弧菌诱导后,收集耳鲍血淋巴,利用微量测定法进行抗菌活性分析.结果发现,耳鲍血淋巴具有抗革兰氏阴性菌-溶藻弧菌、副溶血弧菌、河流弧菌、嗜水气单胞菌、大肠杆菌的活性,对革兰氏阳性菌的抑制活性较低.利用胰蛋白酶对耳鲍血淋巴水解后,其抗菌活性消失,表明其中抗菌成分为蛋白质.将耳鲍血淋巴在不同温度(37～95 ℃)和不同pH(2.5～5.0)下保温,发现其抗菌活性成分对热及酸耐受性较强.耳鲍血淋巴在低浓度无溶血活性.该抗菌组分在耳鲍的免疫防御中具有一定的作用.
|
| 关 键 词： | 耳鲍 血淋巴 抗菌肽 |
Antimicrobial Activity in Hemolymph from Abalone Haliotis asinina Linnaeus |
| |
| ZHANG Ying-xia,MANCHU Ri-ga.Antimicrobial Activity in Hemolymph from Abalone Haliotis asinina Linnaeus[J].Fisheries Science,2009,28(5). |
| |
| Authors: | ZHANG Ying-xia MANCHU Ri-ga |
| |
| Institution: | 1.Key Laboratory of Tropic Biological Resources;Ministry of Education;Hainan Key Laboratory of Tropical Hydrobiology Technology;College of Marine Science;Hainan University;Haikou 570228;China;2.College of Agriculture;China |
| |
| Abstract: | Antimicrobial activity was determined in hemolymph from abalone Haliotis asinina Linnaeus challenged by bacterium Vibrio alginolyticus by miniaturized methods.There were antibacterial activities for the Gram negative bacteria V.alginolyticus,V.parahaemolyticus,V.fluvialis,Aeromonas hydrophila and Escherichia coli,and weak antibacterial activity for the Gram positive bacteria Staphylococcus aureus and Bacillus subtilis in the hemolymph.The hemolymph had no antibacterial activity when it was digested with a p... |
| |
| Keywords: | Haliotis asinina Linnaeus hemolymph antimicrobial peptide |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
