蚯蚓提取物对小鼠生理效应的研究

目的研究蚯蚓蛋白提取物对免疫抑制小鼠肠黏膜免疫功能的影响。方法将30只昆明种小鼠随机分为6组：空白对照组、免疫抑制模型组(CTX)、免疫抑制+高(CTX+EEH)、中(CTX+EEM)、低蚯蚓蛋白剂量组(CTX+EEL)和蚯蚓蛋白组(EE)，对小鼠连续灌胃20 d；第18 天起，CTX、CTX+EEH、CTX+EEM和CTX+EEL组腹腔注射50 mg/kg环磷酰胺，连续注射3 d。计算免疫器官指数，检测肠道细胞因子和黏膜屏障蛋白。结果CTX组胸腺指数、脾脏指数和肠道细胞因子均显著低于空白对照组(P<0.05)。与CTX组相比，CTX+EEH组的脾脏指数、细胞因子TNF-α、IFN-γ和IL-10含量、sIgA含量以及黏膜屏障蛋白ZO-1和Occludin基因表达量均显著升高(P<0.05)。CTX+EEM组的脾脏指数、IL-10含量以及ZO-1、Claudin-1和Occludin基因表达显著高于CTX组(P<0.05)。结论蚯蚓蛋白可改善免疫抑制小鼠肠道黏膜免疫功能，增强肠道免疫屏障。本研究将为蚯蚓蛋白在动物疾病的预防和治疗方面提供理论基础。

Function of mucilaginous secretions in the antibacterial immunity system of Eisenia fetida

PurposeTo study the effects of earthworm protein extract on the intestinal mucosal immune function of immunosuppressed mice. MethodsA total of 30 Kunming mice were randomly divided into six groups: blank control group, immunosuppressive model group (CTX), immunosuppressive+high (CTX+EEH)/medium (CTX+EEM)/low (CTX+EEL) earthworm protein dose group, and earthworm protein group (EE). All groups were gavaged intragastally for consecutive 20 days. On the 18th day, CTX, CTX+EEH, CTX+EEM and CTX+EEL groups were intraperitoneously injected with 50 mg/kg cyclophosphamide for three consecutive days. Immune organ index was calculated, intestinal cytokines and mucosal barrier proteins were detected. ResultsThe thymus index, spleen index and intestinal cytokines in CTX group were significantly lower than those in blank control group (P<0.05). Compared with CTX group, spleen index, contents of cytokines TNF-α, IFN-γ and IL-10, content of sIgA and gene expression of ZO-1 and Occludin in CTX+EEH group were significantly increased (P<0.05). The spleen index, IL-10 content and gene expression of mucosal barrier proteins ZO-1, Claudin-1 and Occludin in CTX+EEM group were significantly higher than those in CTX group (P<0.05). ConclusionEarthworm protein can improve the intestinal mucosal immune function and enhance the intestinal immune barrier in immunosuppressed mice. This study will provide a theoretical basis for earthworm protein in the prevention and treatment of animal diseases.