中国小龙虾产业发展报告 (2022)

采用液相色谱-质谱联用非靶向代谢组学方法，研究了两种日粮饲喂下克氏原螯虾 (Procambarus clarkii) 肌肉代谢物的差异及变化，为提升其养殖品质提供参考。通过样本主成分分析 (Principal components analysis, PCA)，并与基因组百科全书数据库 (Kyoto Encyclopedia of Genes and Genomes, KEGG) 进行比对，筛选出虾肉中的差异代谢物并分析原因。结果显示：通过代谢组学分析，在正离子和负离子模式下共筛选出27种显著差异代谢物；与常规饲料相比，发酵饲料实验组中腺苷酸基琥珀酸、丝氨酸、磷酯酰胆碱、奎尼酸、补骨脂素、磷酯酰丝氨酸、谷氨酸等显著增加；经KEGG通路分析，变化显著的前４条通路分别是组氨酸代谢通路、精氨酸-脯氨酸代谢通路、蛋白质消化与吸收代谢通路和氨酰-tRNA合成通路。研究结果初步表明，日粮在调节克氏原螯虾氨基酸代谢、蛋白质合成和辅助合成氨酰-tRNA酶类等方面起积极作用。

Applications of quantitative metabolomics to revolutionize early diagnosis of inborn errors of metabolism in India

To provide references for improving the quality of crayfish (Procambarus clarkii) meat, we studied the differences in muscle metabolites of crayfish fed with two diets by a non-targeted metabolomic method using liquid chromatography mass spectrometry. Different metabolites in meat were screened by principal components analysis (PCA), cluster analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. The results show that 27 significantly different metabolites were screened under the positive and negative ion modes. Compared with the control group (General feed), the contents of adenylsuccinic acid, phosphatidylcholine, quinate, psoralen, phosphatidylserine and glutamic in the experimental group (Fermented feed) increased significantly. According to KEGG pathway analysis, the top four pathways with the highest concentration of metabolites were histidine metabolism pathway, arginine proline metabolism pathway, protein digestion and absorption metabolism pathway, as well as -tRNA synthesis pathway. The results indicate that diets play a positive role in regulating amino acid metabolism, protein synthesis and assisting in the synthesis of aminoacyl-tRNA enzymes in organisms.