利用前列腺癌细胞株比较各种茄红素的传送载体对茄红素的稳定性及吸收效果

许多研究指出摄取富含茄红素的食物具有降低多种癌症的发生率,如前列腺癌、肺癌等1,2]。在茄红素体外试验中,最常使用四氢呋喃(tetrahydrofuran;THF)作为溶剂,但THF结构不稳定,极易氧化,且具有细胞毒性,因此易造成茄红素体外试验之干扰3￣5]。本实验拟使用胎牛血清(fetalbovineserum;FBS)作为载体,我们认为FBS中的脂蛋白(lipoprotein)可以增加茄红素稳定性并促进细胞的吸收效果。

Effects of delivery vehicle on the uptake and stability of lycopene in human prostate cancer cells

The recent epidemiological studies suggest that lycopene may have chemopreventive effects against several types of cancer including prostate cancer and lung cancer,etc Cell culture studies are useful for elucidating the mechanisms of action of lycopene,and tetrahydrofuran(THF)has commonly been used to deliver lycopene to cells. However,there are several disadvantages associated with the use of tetrahydrofuran,and cytotoxicity because of autoxidation. Here,we used fetal bovine serum(FBS)as the delivery vehicle for lycopene,and the studies indicated that the lipoprotein of FBS was likely responsible for the improved stability and cellular uptake of lycopene.