| 家蚕耐热近等基因系的构建及分子标记育种技术的开发应用 |
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| 引用本文： | 赵远,吴阳春,张健.家蚕耐热近等基因系的构建及分子标记育种技术的开发应用[J].安徽农业科学,2009,37(36). |
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| 作者姓名： | 赵远 吴阳春 张健 |
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| 作者单位： | 江苏科技大学生物与环境工程学院,江苏镇江,212018;中国农业科学院蚕业研究所,江苏镇江,212018;中国农业科学院蚕业研究所,江苏镇江,212018 |
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| 基金项目： | 江苏高技术项目，江苏科技大学博士基金 |
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| 摘 要： | 目的]为家蚕近等基因系分子标记育种技术的开发利用提供理论依据。方法]采用构建近等基因系的方法,连续回交,将耐热基因导入到敏感品种欧_(17)中,经过6代回交,耐热辅助选择,2代自交。结果]获得了具有耐热基因的家蚕近等基因系(新种质)。同时监测了各回交后代的抗性水平,测定了近等基因系的分子标记,并利用SSR标记与耐热基因连锁的品种尝试耐热实用品种的杂交种组配。结论]成功构建了耐热近等基因系,监测了回交后代的抗性水平、饲养成绩,并测定了近等基因系的分子标记。
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| 关 键 词： | 蚕品种 培育 近等基因系 SSR分子标记 |
Production of Bombyx mori Thermotolerance Near-isogenic Lines and Development and Application of Molecular Marker Breeding Technique |
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| ZHAO Yuan et al.Production of Bombyx mori Thermotolerance Near-isogenic Lines and Development and Application of Molecular Marker Breeding Technique[J].Journal of Anhui Agricultural Sciences,2009,37(36). |
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| Authors: | ZHAO Yuan |
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| Institution: | ZHAO Yuan et al(College of Biological , Envronmental Engineering,Jiangsu University ot Science , technology,Zhenjiang,Jiangsu 212018) |
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| Abstract: | Objective]The study aimed to provide theoretical basis for development and application of molecular marker breeding technique to obtain Bombyx mori near-isogenic lines(NILs).Method]Thermotolerance gene was introduced into sensitive variety Ou17 by developing NILs and recurrent backcross,then through six generations of backcross,thermotolerance-assisted selection,and two generations of self-cross. Result]Bombyx mori NILs carrying thermotolerance gene(new germplasm) were produced.Meanwhile,thermotolerance ... |
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| Keywords: | Silkworm species Cultivation Near-isogenic lines SSR molecular marker |
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