MHC-linked protection from diabetes dissociated from clonal deletion of T cells |
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Authors: | J B?hme B Schuhbaur O Kanagawa C Benoist D Mathis |
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Institution: | Laboratoire de Genetique Moléculaire des Eucaryotes du CNRS, Faculté de Médecine, Strasbourg, France. |
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Abstract: | The I-E molecule of the major histocompatibility complex (MHC) can prevent the spontaneous development of diabetes in nonobese diabetic (NOD) mice. The mechanism of this protection has been investigated by breeding wild-type and promoter-mutated E kappa alpha transgenes onto the NOD genetic background. Animals carrying the various mutated transgenes expressed I-E on different subsets of immunocompetent cells, and thus cells important for the I-E protective effect could be identified. Although the wild-type transgene prevented the infiltration of lymphocytes into pancreatic islets, none of the mutants did. However, all of the transgenes could mediate the intrathymic elimination of T cells bearing antigen receptors with variable regions that recognize I-E. Thus, the I-E molecule does not protect NOD mice from diabetes simply by inducing the deletion of self-reactive T cells. |
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