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生防细菌L5生防相关因子的初步分析及其种类鉴定
引用本文:钱兰娟,李倩,张清霞,童蕴慧,徐敬友. 生防细菌L5生防相关因子的初步分析及其种类鉴定[J]. 植物病理学报, 2011, 41(3): 295-330
作者姓名:钱兰娟  李倩  张清霞  童蕴慧  徐敬友
作者单位:扬州大学园艺与植物保护学院,扬州,225009
基金项目:国家自然科学基金资助项目,江苏省高校自然基金资助项目,扬州大学大学生科技创新基金资助项目
摘    要:从扬州郊区土壤中分离到细菌菌株L5,该菌株抑菌谱广,对多种病原真菌都有明显的拮抗能力,离体叶片生物测定结果表明菌株L5对番茄灰霉病防效达72%.对该菌株的生防相关性状进行分析,结果表明菌株L5可产生硝吡咯菌素(Pyrrolnitrin)和嗜铁素等抗菌物质.经过16S rDNA序列、16S-23S rDNA间区序列同源性分析和生理生化性状的测定,将该菌株鉴定为气味沙雷氏菌(Serratia odorifera).细菌菌株L5包含2个16S-23S rDNA的间区(IGSs),其中IGS1内含tRNA Glu基因,间区类型属于IGSG;IGS2内含tRNA lle和tRNA Aia基因,间区类型属于IGS LA.

关 键 词:16S rDNA  16S-23S rDNA间区  沙雷氏菌  硝吡咯菌素  番茄灰霉病菌
收稿时间:2010-07-12;

Characterization and identification of a biocontrol bacterial strain L5
QIAN Lan-juan,LI Qian,ZHANG Qing-xia,TONG Yun-hui,XU Jing-you. Characterization and identification of a biocontrol bacterial strain L5[J]. Acta Phytopathologica Sinica, 2011, 41(3): 295-330
Authors:QIAN Lan-juan  LI Qian  ZHANG Qing-xia  TONG Yun-hui  XU Jing-you
Affiliation:QIAN Lan-juan,LI Qian,ZHANG Qing-xia,TONG Yun-hui,XU Jing-you(College of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,China)
Abstract:Bacterial strain L5 was isolated from soil in Yangzhou and showed inhibitory effect to many pathogenic fungi in dual-culture tests.This strain produced pyrrolnitrin and siderphore,and showed 72% efficiency to control grey mould caused by Botrytis cinerea on detached tomato leaves.The strain L5 was identified as Serratia odorifera based on sequence analysis of 16S rDNA and 16S-23S rDNA intergenic spacers(IGS),physiological and biochemical characterization.The analysis of IGS sequence found two different type...
Keywords:16S rDNA  16S-23S rDNA IGS  Serratia  pyrrolnitrin  Botrytis cinerea  
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