| 生防细菌L5生防相关因子的初步分析及其种类鉴定 |
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| 引用本文： | 钱兰娟,李倩,张清霞,童蕴慧,徐敬友.生防细菌L5生防相关因子的初步分析及其种类鉴定[J].植物病理学报,2011,41(3):295-330. |
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| 作者姓名： | 钱兰娟 李倩 张清霞 童蕴慧 徐敬友 |
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| 作者单位： | 扬州大学园艺与植物保护学院,扬州,225009 |
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| 基金项目： | 国家自然科学基金资助项目，江苏省高校自然基金资助项目，扬州大学大学生科技创新基金资助项目 |
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| 摘 要： | 从扬州郊区土壤中分离到细菌菌株L5,该菌株抑菌谱广,对多种病原真菌都有明显的拮抗能力,离体叶片生物测定结果表明菌株L5对番茄灰霉病防效达72%.对该菌株的生防相关性状进行分析,结果表明菌株L5可产生硝吡咯菌素(Pyrrolnitrin)和嗜铁素等抗菌物质.经过16S rDNA序列、16S-23S rDNA间区序列同源性分析和生理生化性状的测定,将该菌株鉴定为气味沙雷氏菌(Serratia odorifera).细菌菌株L5包含2个16S-23S rDNA的间区(IGSs),其中IGS1内含tRNA Glu基因,间区类型属于IGSG;IGS2内含tRNA lle和tRNA Aia基因,间区类型属于IGS LA.
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| 关 键 词： | 16S rDNA 16S-23S rDNA间区 沙雷氏菌 硝吡咯菌素 番茄灰霉病菌 |
| 收稿时间： | 2010-07-12; |
Characterization and identification of a biocontrol bacterial strain L5 |
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| QIAN Lan-juan,LI Qian,ZHANG Qing-xia,TONG Yun-hui,XU Jing-you.Characterization and identification of a biocontrol bacterial strain L5[J].Acta Phytopathologica Sinica,2011,41(3):295-330. |
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| Authors: | QIAN Lan-juan LI Qian ZHANG Qing-xia TONG Yun-hui XU Jing-you |
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| Institution: | QIAN Lan-juan,LI Qian,ZHANG Qing-xia,TONG Yun-hui,XU Jing-you(College of Horticulture and Plant Protection,Yangzhou University,Yangzhou 225009,China) |
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| Abstract: | Bacterial strain L5 was isolated from soil in Yangzhou and showed inhibitory effect to many pathogenic fungi in dual-culture tests.This strain produced pyrrolnitrin and siderphore,and showed 72% efficiency to control grey mould caused by Botrytis cinerea on detached tomato leaves.The strain L5 was identified as Serratia odorifera based on sequence analysis of 16S rDNA and 16S-23S rDNA intergenic spacers(IGS),physiological and biochemical characterization.The analysis of IGS sequence found two different type... |
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| Keywords: | 16S rDNA 16S-23S rDNA IGS Serratia pyrrolnitrin Botrytis cinerea |
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