玉米异交不亲和基因Ga1-S的蛋白质组分析

为了研究玉米异交不亲和的蛋白表达机制，以玉米(Zea mays L.)异交不亲和基因Gal-S的一对近等基因系W22(GG)与w22(gg)为材料，组配自交(GG×GG)、正交(gg×GG)与反交(GG×gg) 3个组合。首先采用荧光显微技术, 观察比较了3个组合中花粉管在花丝中的生长过程；其次采用IEF/SDS-PAGE双向凝胶电泳与基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)技术，比较研究了授粉后10 h自交与反交母本W22(GG)花丝蛋白质组的特异表达差异。结果表明，授粉后10 h，自交与正交花粉管伸长可达花丝基部，而反交花丝基部未观察到花粉管；自交与反交母本W22(GG)花丝蛋白质组共检测到25个差异蛋白质点，其中15个在自交中特异表达，10个在反交中特异表达；通过MALDI-TOF-MS质谱测序和MASCOT序列分析，注释了12个蛋白质点；其中自交中特异表达的蛋白质点11、12、14和异交中特异表达的蛋白质点18、22、24可能与玉米异交不亲和密切相关。

Proteomic Analysis of Maize Cross Incompatibility Gene Ga1-S

The objective of this paper was to study the cross incompatibility gene Ga1-S in maize through proteomic approach. Near isogonic lines of Ga1-S gene, W22 (GG) and w22 (gg), were used to make the crosses of GG×GG, gg×GG,and GG×gg respectively. First，we observed the growth process of pollen tubes grown into silks in three crosses by fluorescence microscopy; second, total silk proteins were extracted from silks of the W22 (GG) at 10 h after pollination. Total proteins were extracted by TCA/Acetone, separated by two-dimensional gel electrophoresis (2-DE) and analyzed through MALDI-TOF-MS mass spectrometry. The results indicated that pollen tube couldn’t grow into silks base in the cross of GG×gg, but could in the other two crosses; In the silk proteome of GG×GG and GG×gg , there were 25 differentially expressed proteins, including 15 specifically expressed in GG×GG, and 10 specifically expressed in GG×gg. And 12 of them were annotated in various databases by MALDI-TOF-MS and MASCOT analyses. Proteins 11, 12, 14, 18, 22 and 24 presumably play important roles in the maize cross incompatibility.