| 黄瓜枯萎病菌遗传多样性的AFLP分析 |
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| 引用本文： | 张艳菊,陈霞,刘东,秦智伟,周秀艳,蒲子婧.黄瓜枯萎病菌遗传多样性的AFLP分析[J].植物病理学报,2011,41(3):301-304. |
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| 作者姓名： | 张艳菊 陈霞 刘东 秦智伟 周秀艳 蒲子婧 |
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| 作者单位： | 东北农业大学,哈尔滨,150030 |
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| 基金项目： | 国家科技部863项目，"十一五"国家科技支撑计划项目，东北农业大学创新团队，黑龙江省教育厅科学技术研究项目 |
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| 摘 要： | 黄瓜枯萎病是由半知菌亚门尖孢镰刀菌黄瓜专化型(Fusarium oxysporumf.sp.cucumainum Owen)侵染引起的一种土传病害,是影响黄瓜生产的最主要病害之一1].近年来随着分子生物学技术的迅速发展,国内外学者对于病原真菌的遗传多样性做了大量的研究,Wang等2]对影响黄瓜枯萎病菌AFLP技术体系的多种因素作了探讨,得到了1种适合于黄瓜枯萎病菌AFLP分析的优化体系;Duan等3]应用RAPD、ISSR和AFLP标记揭示出了西瓜枯萎病菌株在分子水平上的遗传多样性.
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| 关 键 词： | 选择性扩增 尖孢镰刀菌 土传病害 菌株 分子标记技术 半知菌亚门 遗传多样性 黄瓜枯萎病菌 |
| 收稿时间： | 2010-05-25; |
AFLP analysis of genetic diversity of Fusarium oxysporum f.Sp.Cucumainum |
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| ZHANG Yan-Ju,CHEN Xia,LIU Dong,QIN Zhi-Wei,ZHOU Xiu-Yan,PU Zi-jing.AFLP analysis of genetic diversity of Fusarium oxysporum f.Sp.Cucumainum[J].Acta Phytopathologica Sinica,2011,41(3):301-304. |
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| Authors: | ZHANG Yan-Ju CHEN Xia LIU Dong QIN Zhi-Wei ZHOU Xiu-Yan PU Zi-jing |
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| Institution: | ZHANG Yan-Ju,CHEN Xia,LIU Dong,QIN Zhi-Wei,ZHOU Xiu-Yan,PU Zi-jing(Northeast Agricultural University,Harbin 150030,China) |
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| Abstract: | The genetic diversity of Fusarium specises isolated from cucumber plants showing wilt disease from 8 cities was detected by amplified restriction fragment polymorphism(AFLP).Sixteen combinations of primer pair were selected from 130 combinations and used to amplify the genomic DNA of 351 Fusarium oxysporum isolates.A total of 116 444 bands were obtained and 60 579 of them showed polymorphism,accounting for 52.0%.The genetic distances of 351 F.oxysporum isolates were ranged 0.56-1.00 based on AFLP markers,av... |
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| Keywords: | cucumber Fusarium oxysporum AFLP genetic diversity |
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