| 爱玉子基因组DNA的提取和质量分析 |
| |
| 引用本文： | 吴文珊,朱晓东,郑翠芳,方丽娜,方玉霖.爱玉子基因组DNA的提取和质量分析[J].福建林业科技,2008,35(3). |
| |
| 作者姓名： | 吴文珊 朱晓东 郑翠芳 方丽娜 方玉霖 |
| |
| 作者单位： | 1. 福建师范大学生命科学学院,福建,福州,350108;福建农林大学生命科学学院,福建,福州,350002
2. 福建师范大学生命科学学院,福建,福州,350108 |
| |
| 基金项目： | 福建省科技厅科技计划，福建省自然科学基金，福建省大学生创新性实验计划项目 |
| |
| 摘 要： | 以爱玉子的叶片为材料,对CTAB分离总DNA的方法进行了改进,即在提取液中加入β-巯基乙醇、PVP,将CTAB的浓度提高到3%,用氯仿-异戊醇取代酚进行抽提。结果表明,改进后的方法可有效地去除杂质,防止材料褐化,获得的DNA样品OD260nm/OD280nm比值为1.8左右,质量好且产率较高,能完全满足SRAP分析及限制性内切酶酶切的要求。
|
| 关 键 词： | 爱玉子 DNA提取 SRAP 限制性内切酶酶切 |
Genomic DNA extraction and quality analysis of Ficus awkeotsang |
| |
| WU Wen-shan,ZHU Xiao-dong,ZHENG Cui-fang,FANG Li-na,FANG Yu-lin.Genomic DNA extraction and quality analysis of Ficus awkeotsang[J].Journal of Fujian Forestry Science and Technology,2008,35(3). |
| |
| Authors: | WU Wen-shan ZHU Xiao-dong ZHENG Cui-fang FANG Li-na FANG Yu-lin |
| |
| Abstract: | This paper explored an improved CTAB method to extract high-quality genomic DNA from the leaves of Ficus awkeotsang Makino,which was the application of β-SH(mercapto) ethanol and PVP,and increasing the concentration of CTAB up to 3%,and substituting chloroform-isoamylol for phenol.The research results showed that this modified method can effectively eliminate impurity and prevent DNA samples from turning brown.High-quality and high-yield genomic DNA with OD260nm/OD280nm approximate 1.8 was obtained,and it was fully suitable for SRAP analysis and restriction enzyme digestion. |
| |
| Keywords: | Ficus awkeotsang Makino DNA extraction SRAP restriction enzyme digestion |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
