Genotype differencies in direct plant regeneration from stem explants ofPopulus tremula in Turkey |
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Authors: | Nermin Gözükirmizi Kas?m Bajroviç Zeliha ?pekçi Melih Boydak Tahsin Akalp Korhan Tunçtaner Hülya Balkan Hulki Tanr?yar Mehmet Çal?ko?lu Tijen O?ra? Öznur Özden Mümtaz Tulukçu Turan Tank |
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Institution: | (1) Tubitak, MRC, Research Institute for Genetic Engineering and Biotechnology, P.O. Box: 21, 41470 Gebze-Kocaeli, Turkey;(2) Faculty of Forestry, University of İstanbul, İstanbul, Turkey;(3) Poplar Research Institute, İzmit-Kocaeli, Turkey;(4) State Owned Paper and Pulp Factory (SEKA), İzmit-Kocaeli, Turkey;(5) Faculty of Science, University of İstanbul, Vezneciler-İstanbul, Turkey |
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Abstract: | Plant regeneration from 9 clones ofPopulus tremula representing diverse genotypes originated from Turkey, has been studied in different media, such as Murashige and Skoog’s
Medium (MS), Aspen Culture Medium (ACM) and Woody Plant Medium (WPM) with appropriate supplements of growth regulators. Regeneration
efficiency on WPM containing 1 mg/l zeatin using stem explantsin vitro, was higher than on MS and ACM. In this system, the time required for shoot regeneration was 2 weeks, which was shorter by
two weeks than that of previous reports. A strong genotype dependence was observed among the tested nine clones. The regeneration
system, we describe here, did not involve the callus phase and thus was less prone to somaclonal variation. When adventitious
microshoots were cultured on WPM supplemented with 0.5 mg/l or 2 mg/l indole-3-butyric acid (IBA), root formation was obtained after 1 week of culture. Adaptation of regenerated plantletsin vivo was 100%. This system for micropropagation ofPopulus tremula is expected to be suitable for studies on transformation of genes involved in lignin biosynthesis via modification of lignin
content.
This study was a part of the Project No. 62-02-001 TUBITAK-MAN-GMBAE named “Investigations on Biogenetically Develop Poplar
Clones as a Raw Material for Pulp and Paper Industry” and financially supported by General Management of Turkish Pulp and
Paper Mills. |
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Keywords: | micropropagation poplar root formation tissue culture |
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