小麦植酸酶基因TaPHY1的克隆、分子特征和表达特性研究

利用小麦植酸酶基因PAPhya1进行同源查找,获得1个与该基因高度同源、尚未开展分子特征和生物学功能研究的小麦新型植酸酶基因TaPHY1.TaPHY1的全长cDNA序列为1771 bp,编码548个氨基酸残基,TaPHY1含有紫色酸性磷酸酶(PAP)类植酸酶通常含有的保守域Metallophos、Purple acid...

Cloning, molecular characterization, and expression pattern of TaPHY1, a novel phytase gene in wheat(Triticum aestivum L.)

Using a wheat purple acid phosphatase(PAP)-type phytase gene referred to PAPhya1 as a query,a novel phytase gene to be homologous to PAPhya1 that has not been characterized and functionally analyzed was identified and designated as TaPHY1 in this study.The full length of TaPHY1 is 1771 bp,encoding a 548-aa polypeptide.TaPHY1 contains three conserved signature domains generally identified in PAP-type phytases,including metallophos,purple acid phosphatase and metallo-dependent phosphatases.Phylogenetic analysis revealed that TaPHY1 shares high similarities to the previously reported phyase genes such as PAPhya1～PAPPhya3 and PAPhyb1～PAPhyb2,as well as high similarities to PAP-type phytase genes in other plant species including the barley(H.vulgare) and rye(S.cereale).TaPHY1 is abundantly expressed in germinating seed and little expressed in the primary roots and leaves.The expressed TaPHY1 based on the prokaryotic expression system exhibited strong capability to degrade the phytate,a major component of organic phosphate compound in the mature seed.Taken together,as a novel PAP-type phytase gene in wheat,TaPHY1 plays crucial roles on degradation of the seed phytic acid and its derivatives,and provides the Pi for the novel organs of the young seedlings.