生长延缓剂延长苹果组培苗继代间隔的保护酶机制

以‘富士’(Malus domestica‘Fuji')、‘乔纳金’(M.‘Jonagold')苹果试管苗为试材,研究了比久(丁酰肼,B9)、矮壮素(CCC)、多效唑(PP333)等生长延缓剂处理下苹果组培苗延缓生长以及恢复生长后SOD、POD活性和MDA含量的变化,以期为筛选苹果组培苗延缓生长种质保存的适宜方法提供理...

Mechanism in protective enzyme of plant growth retardant on prolonging subculture interval in apple in vitro

This paper explored the SOD and POD activity and MDA amount of 'Fuji' and 'Jonagold' apple explants in vitro during conservation period with B9,CCC,and PP333 supplied in subculture media,and at the stage after recovery to the normal growth status,in order to find optimal methods for germplasm preservation.The results showed that the SOD and POD activity increased and the MDA amount decreased with B9,CCC,and PP333 supplied in subculture media.The efficacy varied with the concentration supplied and the significant difference was found when the concentration reached 100-120 mg/L of B9,50-75 mg/L of CCC,or 3-4 mg/L of PP333.There was no significant difference among treatments in terms of the SOD and POD activity and MDA amount at the first subculture period after recovery from retardant growth preservation.It demonstrated that the protective enzymes could mitigate injury caused by stress to a certain extent and slow down aging process.The plantlets of 'Fuji' responded faster to the treatments in terms of the changing of SOD and POD activity and MDA amount,and showed better efficacy compared to that of 'Jonagold'.