| 西兰花籽中完形硫代葡萄糖苷的HPLC-MS-MS鉴定 |
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| 引用本文： | 杨艳婧,张学武. 西兰花籽中完形硫代葡萄糖苷的HPLC-MS-MS鉴定[J]. 安徽农业科学, 2009, 37(34): 16754-16756 |
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| 作者姓名： | 杨艳婧 张学武 |
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| 作者单位： | 华南理工大学轻工与食品学院,广东广州,510640;华南理工大学轻工与食品学院,广东广州,510640 |
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| 摘 要： | [目的]建立一种简单、快速、经济的鉴定蔬菜中硫代葡萄糖苷的方法。[方法]用70％甲醇从西兰花籽中提取硫代葡萄糖苷，以甲酸作为离子调节剂，采用梯度洗脱的方法对提取物进行分离，并采用液相色谱-质谱-质谱联用技术对提取物成分进行鉴定。[结果]从西兰花籽中成功鉴定出8种硫代葡萄糖苷，其保留时间分别为5．9、7．7、8．8、9．5、13．6、16．0、19．3、28．6min，分子量分别为422、468、438、436、372、406、420、402；该鉴定方法所测样品不需经过脱硫等预处理，大大节省了试验时间，且样品需要量较少，试验效率较高。[结论]该研究建立了一种鉴定蔬菜中硫代葡萄糖苷的新方法。
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| 关 键 词： | 西兰花籽 硫代葡萄糖苷 HPLC-MS-MS |
Identification on Intact Glucosinolates in Broccoli Seeds by HPLC-MS-MS |
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| YANG Yan-jing et al. Identification on Intact Glucosinolates in Broccoli Seeds by HPLC-MS-MS[J]. Journal of Anhui Agricultural Sciences, 2009, 37(34): 16754-16756 |
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| Authors: | YANG Yan-jing et al |
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| Affiliation: | YANG Yan-jing et al(College of Light Industry , Food Science,South China University of Technology,Guangzhou,Guangdong 510640) |
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| Abstract: | [Objective] The study was to establish a sample,rapid and economic identification method on glucosinolates in vegetables.[Method] The glucosinolates in broccoli seeds were extracted by 70% methanol,and the extracts were separated by gradient elution method with formic acid as regulator,and the compositions of the extracts were identified by HPLC-MS-MS.[Result] 8 kinds of Glucosinolate in broccoli seeds were successfully identified,and their retention times were 5.9,7.7,8.8,9.5,13.6,16.0,19.3,28.6 min resp.m... |
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| Keywords: | HPLC-MS-MS Broccoli seeds Glucosinolates HPLC-MS-MS |
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