Application of a whole-blood lymphocyte stimulation test in detection of Brucella infection in an outbreak |
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| Authors: | J. M. B. Kaneene E. D. Sloane D. W. Johnson R. K. Anderson |
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| Affiliation: | (1) Department of Large Animal Surgery and Medicine, College of Veterinary Medicine, Michigan State University, 48824 East Lansing, Michigan, (U.S.A.);(2) Department of Large Animal Clinical Sciences, College of Veterinary Medicine, University of Minnesota, 55108 St. Paul, Minnesota, (U.S.A.) |
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| Abstract: | A whole-blood lymphocyte stimulation test (WBLST), the standard plate and tube agglutination tests, the Brucella buffered antigen test, the 2-mercaptoethanol agglutination test, the Rivanol plate precipitation test and bacteriological isolation were utilized in a brucellosis outbreak investigation in a beef herd. Three of the animals were classified as not infected serologically. However, these 3 animals were classified as infected on the WBLST, andBrucella abortus biotype 1 (not strain 19) was isolated from their lymph nodes. The WBLST exhibited significant sensitivity in this investigation and more observations of this nature might strengthen the application of this assay in similar situations. |
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