| RT-PCR鉴别小反刍兽疫病毒疫苗株与强毒株方法的建立 |
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| 引用本文： | 张玲,包静月,李林,王志亮. RT-PCR鉴别小反刍兽疫病毒疫苗株与强毒株方法的建立[J]. 动物医学进展, 2010, 31(3): 17-20 |
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| 作者姓名： | 张玲 包静月 李林 王志亮 |
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| 作者单位： | 青岛农业大学动物科技学院,山东青岛,266109;中国动物卫生与流行病学中心国家外来动物疫病诊断中心,山东青岛,266032;青岛农业大学动物科技学院,山东青岛,266109 |
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| 基金项目： | 农业部948项目(2006-G57(3)) |
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| 摘 要： | 根据GenBank中公布的小反刍兽疫病毒的H基因或全基因序列,利用软件Primer Premier5.0设计引物,扩增片段长度为477bp,建立区分小反刍兽疫疫苗毒与基因4系野毒的RT-PCR检测方法,并用于临床检测。结果表明,建立的RT-PCR鉴别诊断方法能特异性区分疫苗株Nigeria75/1与基因4系PPRV,与基因3系、牛瘟病毒、犬瘟热病毒等同属病毒无交叉反应;最低可以检测到浓度为7.7×10-5ng/μL的RNA模板;该方法操作简单,耗时短,可以初步用于临床鉴别诊断。
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| 关 键 词： | RT-PCR 鉴别 小反刍兽疫病毒 |
Establishment of RT-PCR Assay for Differentiation of Vaccine Strain and Wild-type Peste des Petits Ruminants Virus |
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| ZHANG Ling,BAO Jing-yue,LI Lin,WANG Zhi-liang. Establishment of RT-PCR Assay for Differentiation of Vaccine Strain and Wild-type Peste des Petits Ruminants Virus[J]. Progress In Veterinary Medicine, 2010, 31(3): 17-20 |
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| Authors: | ZHANG Ling BAO Jing-yue LI Lin WANG Zhi-liang |
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| Affiliation: | 1.College of Animal Science and Technology;Qingdao Agricultural University;Qingdao;Shandong;266109;China;2.National Diagnostic Center for Exotic Animal Diseases;China Animal Health and Epidemiology Center;266032;China |
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| Abstract: | Based on the H gene sequence of peste des petits ruminants virus(PPRV)published in GenBank,a pair of special primers were designed by Primer Premier 5.0,the fragment of amplification was 477 bp in length.The establishment of RT-PCR assay aimed to differentiate vaccine and field isolates of 4-type peste des petits ruminants virus.By using the one-step RT-PCR,the wild-type and vaccine strains of PPRV in clinical samples were detected and accurately distinguished.The detection limit concentration of RNA was 7.... |
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| Keywords: | RT-PCR |
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