枯草芽孢杆菌wlcl芽孢生成条件优化及净水作用研究

目的]提高枯草芽孢杆菌的产量并降低生产成本。方法]利用旋转回归法研究豆饼粉、葡萄糖对枯草芽孢杆菌（Bacillus subti-lis）液体发酵生产芽孢的影响。结果]芽孢形成率与豆饼粉和葡萄糖的回归方程为：Y1=35.8-2.185 65X1＋0.685 666X2-5.962 504X1X1-1.25X1X2-3.462 499X2X2,其因变量和自变量之间的线性关系显著,决定系数为86.21%。豆饼粉为1.2%,葡萄糖为1.0%时,芽孢形成率最大,估计为89%。试验池的COD值从第1天的9.85 mg/L下降到第11天的7.87 mg/L,而对照池的COD值从第1天的8.95 mg/L上升到第7天的9.69 mg/L后再下降。试验池中亚硝酸盐浓度从0.088 mg/L下降到0.059 mg/L,而对照池中亚硝酸盐浓度从0.072 mg/L上升到0.085 mg/L。试验池水体pH值在7.5-8.4的范围内变动,而对照池水体pH值普遍高于试验池,最高达8.7。对照池中H2S浓度在0.008-0.030 mg/L的范围内,而试验池水中H2S浓度从试验初期的0.060 mg/L下降到第11天的0.015 mg/L。结论]培养基中的碳源和氮源是芽孢生成的重要因素。

Study on the Optimization of Formation Condition of Bacillus subtilis wlcl Spore and Its Water Purification Effect

Objective] The purpose was to enhance the yield of Bacillus subtilis and reduce its production cost.Method] The influences of powder of soybean cake and glucose on producing spore by liquid fermentation with B.subtilis were studied by rotary regression design.Result] The regression equation between formation rate of spore and powder of soybean cake,glucose was Y1=35.8-2.185 65X1+0.685 666X2-5.962 504X1X1-1.25X1X2-3.462 499X2X2,the linear correlation between its dependent variable and independent variable...