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Wolbachia在短管赤眼蜂和拟澳洲赤眼蜂种间的水平传递
引用本文:潘雪红,何余容. Wolbachia在短管赤眼蜂和拟澳洲赤眼蜂种间的水平传递[J]. 西北农业学报, 2011, 20(4): 186-188
作者姓名:潘雪红  何余容
作者单位:1. 广西甘蔗研究所,南宁530007;华南农业大学昆虫生态室,广州510640
2. 华南农业大学昆虫生态室,广州,510640
基金项目:国家自然科学基金资助项目
摘    要:用米蛾卵作为短管赤眼蜂和拟澳洲赤眼蜂共寄生的寄主,实现Wolbachia在赤眼蜂种间的水平传递.对Wolbachia的wsp基因进行PCR检测,结果表明,这4头处女雌蜂体内有Wolbachia的存在,且在由这4头处女雌蜂为单系所建立第1~5代拟澳洲赤眼蜂群体内,Wolbaccha的检测均为阳性;wsp基因序列比对和同源性分析结果表明,拟澳洲赤眼蜂和短管赤眼蜂体内Wolbachia的wsp基因序列完全一致.证明未感染Wolbachia的拟澳洲赤眼蜂和感染Wolbachia的短管赤眼蜂在同一米蛾卵体内共同发育时,Wolbachia能从供体短管赤眼蜂成功地水平传递到新宿主拟澳洲赤眼蜂体内,并能在新宿主体内垂直传递5代.

关 键 词:赤眼蜂  沃尔巴克氏体  产雌孤雌生殖  水平传递

Horizontal Transfer of Wolbachia between Trichogramma pretiosum and T. confusum
PAN Xuehong and HE Yurong. Horizontal Transfer of Wolbachia between Trichogramma pretiosum and T. confusum [J]. Acta Agriculturae Boreali-occidentalis Sinica, 2011, 20(4): 186-188
Authors:PAN Xuehong and HE Yurong
Affiliation:PAN Xuehong1,2 and HE Yurong2(1.Guangxi Sugarcane Research Institute,Nanning 530007,China,2.Lab.of Insect Ecology,South China Agricultural University,Guangzhou 510640,China)
Abstract:In this paper,eggs of Corcyra cephalonica Stainton were used as host eggs for synparasitism of Wolbachia-doner T.pretiosum and Wolbachia-recipient T.confusum.Interspecific horizontal transfer of Wolbachia between Trichogramma wasps was proved successfully.Wolbachia was detected by PCR of wsp gene of Wolbachia for up to 5 generations after transferring.The wsp sequence of Wolbachia in new hosts T.confusum was identical to that of T.pretiosum.Therefore,Wolbachia from an infected species,T.pretiosum,were succe...
Keywords:Trichogramma  Wolbachia  Thelytokous parthenogenesis  Horizontal transfer  
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