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| 地生兰组培快繁技术研究 | |
| 引用本文： | 伍建榕,韩素芬,朱有勇,吕梅,王光平,刘婷婷,孙会林.地生兰组培快繁技术研究[J].北方园艺,2008(5):202-205. |
| 作者姓名： | 伍建榕 韩素芬 朱有勇 吕梅 王光平 刘婷婷 孙会林 |
| 作者单位： | 1. 西南林学院,西南生物多样性保育国家林业局重点实验室,云南,昆明,650224 2. 南京林业大学森林资源与环境学院,南京,210037 3. 云南农业大学,植物病理重点实验室,云南,昆明,650201 |
| 基金项目： | 国家自然科学基金，云南省教育厅资助项目 |
| 摘 要： | 利用地生兰蒴果生产种子苗及切取优良母株的侧芽进行茎尖诱导繁殖,筛选出适合云南几种地生兰种子生长和茎尖培育及分生苗生长的培养基配方和组培快繁技术.适合种子发芽培养基为1/2 MS NAA 0.3 mg/L IBA 3.0 mg/L 活性炭(AC)2 g/L 1 g/L兰花培养液(泰国购入);茎尖培养基配方为1/2 MS NAA 0.4 mg/L 6-BA 3.0 mg/L 活性炭(AC)2 g/L 新鲜椰子汁(CM)250 mL/L 1%葡萄糖 微量维生素C,平均诱导原球茎个数3.9/个;丛生芽的诱导1/2 MS NAA 0.4 mg/L 6-BA 4.0 mg/L AC 2 g/L;丛生芽生根1/2 MS NAA 0.2 mg/L AC 2 g/L. |
| 关 键 词： | 地生兰 蒴果 组织培养 生根诱导 |
| 文章编号： | 1001-0009(2008)05-0202-04 |
| 修稿时间： | 2007年12月14 |
Study on the Quick Multiplication of Terrestrial Orchid |
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| WU Jian-rong,HAN Su-fen,ZHU You-yong,LV Mei,WANG Guang-ping,LIU Ting-ting,SUN Hui-lin.Study on the Quick Multiplication of Terrestrial Orchid[J].Northern Horticulture,2008(5):202-205. | |
| Authors: | WU Jian-rong HAN Su-fen ZHU You-yong LV Mei WANG Guang-ping LIU Ting-ting SUN Hui-lin |
| Abstract: | The seeds and the top of stalk of terrestrial orchid as explants were cultured on medium.The tissue culture of terrestrial orchid were studied.The results were as follows: The best culture medium for seed germinate of terrestrial orchid was 1/2 MS NAA 0.3 mg/L IBA 3.0 mg/L active carbon(AC) 2 g/L 1 g/L(culture liquid of the orchifd from Thailand);The best medium in inducing protocorm was 1/2 MS NAA 0.4 mg/L 6-BA 3.0 mg/L (AC) 2 g/L fresh coconut juice(CM) 250 mL/L 1% glucose micro-vitamin C,The best medium in adventitious buds induced was 1/2 MS NAA 0.4 mg/L 6-BA 4.0 mg/L AC 2 g/L;The best medium in rooting of adventitious buds was 1/2 MS NAA 0.2 mg/L AC 2 g/L. |
| Keywords: | Terrestrial orchid Capsule Tissue culture Rooting induction |
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