秀山灰毡毛忍冬中绿原酸、提取工艺的研究

目的]优选灰毡毛忍冬中绿原酸的最佳提取工艺。方法]以浸膏得率和绿原酸含量为考察指标,采用L9(34)正交试验方法优选重庆秀山灰毡毛忍冬水提工艺,并采用UV和HPLC测定绿原酸的含量。结果]以总浸膏得率为指标:15倍水量、提取2次、提取温度85℃、每次30 min;以绿原酸为指标:15倍水量提取2次、提取温度80℃、每次30 min。结论]该工艺简便、快捷稳定,可为灰毡毛忍冬质量控制提供实验依据。

Study on Optimum Extraction Process for Chlorogenic Acid from Lonicera macranthoides Hand. Mazz.of Xiushan

Objective]The aim was to study the optimum extracting progress of chlorogenic acid from Lonicera macranthoides Hand.-Mazz.Method] The optimum extracting condition was investigated by L9(34) orthogonal design with the extraction yield and chlorogenic acid as index.Two different ways of UV and HPLC were carried out for the quantitative and qualitative determination of chlorogenic acid.Result] The total extract yield was taken as index: 15 folds of water,2 times extraction,extraction temperature 85 ℃ and 30 min.The chlorogenic acid was taken as index: 15 folds of water,2 times extraction,extraction temperature 80 ℃ and 30 min.Conclusion] This technology was simple,rapid and stable,and could be used to control the quality of Lonicera macranthoides Hand.-Mazz.