亳菊的组织培养快繁技术研究

目的]探讨亳菊组织培养的快繁技术。方法]以亳菊的茎尖作为外植体,以MS为基本培养基,附加不同剂量的NAA和6-BA,分别进行丛生芽诱导、增殖和生根培养,考察不同激素配比的培养基对亳菊丛生芽形成与生长的影响,以及对试管苗长势和生根的影响,并根据丛生芽的数量、长势、苗高、根条数、繁殖周期等指标,筛选适合亳菊茎尖离体组织培养的培养基配方。结果]亳菊诱导分化的最佳培养基配方为MS＋6-BA0.50 mg/L＋NAA0.10 mg/L;增殖培养的最佳培养基配方为MS全量培养基;诱导生根的最佳配方为1/2MS＋IBA0.30 mg/L。将试管苗移栽到土∶泥炭∶珍珠岩为1∶1∶1的基质中,其成活率达99%,大田移栽的试管苗成活率可高达97.9%。结论]该研究所筛选的培养基配方可作为亳菊脱毒培养的最优技术方案。

Study on Rapid Propagation Technology for Tissue Culture of Dendranthema morifolium ( Ramat.) Tzvel.'Boju' cv. nov.

Objective]The study aimed to discuss the rapid propagation technology for tissue culture of Dendranthema morifolium(Ramat.) Tzvel.'Boju' cv.nov.Method]With the stem tips of D.morifolium as the explants,MS media were taken as the basic media that was added by NAA and 6-BA with different content,to make the tissue cultures of budding induction,multiplication and rooting.The effects of the media with different hormone matching on the form and growth of the clustered buts of D.morifolium and on the growth vigor and rooting of test tube seedlings were investigated and the medium formulas suitable for the tissue culture in vitro on the stem tip of D.morifolium were screened according to the indexes such as the number of clustered buts,growth vigor,seedling height,root number and reproductive cycle.Result]The optimum medium formula for the induction and differentiation of D.morifolium was MS+6-BA 0.50 mg/L+NAA 0.10 mg/L,that for the multiplication culture was MS medium without any hormone and that for rooting induction was 1/2MS+IBA 0.30 mg/L.The survival rate of the test tube seedlings was 99% when being transplanted to the matrix of soil,peat and perlite with the ratio of 1∶1∶1 and was 97.9% when being transplanted to the filed.Conclusion]The medium formulas screened by this research could be taken as the most superior technique program for the virus-free culture of D.morifolium.