Extraction,molecular fractionation and enzyme degradation of organically associated phosphorus in soil solutions |
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Authors: | H. K. Pant A. C. Edwards D. Vaughan |
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Affiliation: | (1) The Macaulay Land Use Research Institute, Craigiebuckler, AB9 2QJ Aberdeen, UK;(2) Present address: Nepal-UK Forestry Research Project, Katmandu, Nepal |
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Abstract: | The concentrations and chemical composition of water-extractable P were compared in four soil types from NE Scotland. All sites were sampled during the early establishment phase of a spring barley (Hordeum vulgare) crop. The quantity of total soluble P extracted ranged from <2.0 to 10 mg P kg soil-1, of which up to 50% was classified as being organically associated. Sample fractionation showed that both orthophosphate inorganic P and organic P were associated with a wide molecular-size range of organic material. A strong positive correlation was readily apparent between P and the sum of Fe + Al in the fractionated samples. The extent of enzymatic hydrolysis of organic P varied between soil samples and the type of enzyme. Phytase consistently produced the greatest degree of hydrolysis. |
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Keywords: | Phosphorus Fractionation Phosphatase Hydrolysis Organic phosphorus Hordeum vulgare |
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