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裙带菜凝集素对鲫的免疫诱导
引用本文:张静,李丹彤,范庆华,邢殿楼,刘靖,姜川. 裙带菜凝集素对鲫的免疫诱导[J]. 大连水产学院学报, 2006, 21(2): 193-195
作者姓名:张静  李丹彤  范庆华  邢殿楼  刘靖  姜川
作者单位:大连水产学院,农业部海洋水产增养殖学与生物技术重点开放实验室,辽宁,大连,116023;华东理工大学,理工优秀生部,上海,200237
基金项目:辽宁省大连市科技局资助项目
摘    要:将从裙带菜Undaria pinnatifida中提取的凝集素作为免疫诱导制剂,注射到鲫Carassius auratusw体内,在连续注射7 d和9 d时,分别对鲫血清中的溶菌酶、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的酶活性进行测定,结果表明,注射凝集素7 d和9 d时,鲫血清中溶菌酶的酶活性比对照组分别提高18.83%、12.69%,SOD的酶活性比对照组分别提高66.67%、139.99%,CAT酶活性比对照组分别提高16.60%、63.63%。说明凝集素可以明显提高鲫血清中这3种酶的表达水平。

关 键 词:裙带菜  凝集素    免疫
文章编号:1000-9957(2006)02-0193-03
收稿时间:2005-04-01
修稿时间:2005-04-01

Induction of sea mustard (Undaria pinnatifida) lectin to immunity system in crucian carp(Carassius auratus)
ZHANG jing,LI dan-tong,FAN qing-hua,XING dian-lou,LIU jing,JIANG Chuan. Induction of sea mustard (Undaria pinnatifida) lectin to immunity system in crucian carp(Carassius auratus)[J]. Journal of Dalian Fisheries University, 2006, 21(2): 193-195
Authors:ZHANG jing  LI dan-tong  FAN qing-hua  XING dian-lou  LIU jing  JIANG Chuan
Abstract:Crucian carp(Carassius auratus) was injected with the lectin extracted from sea mustard Undaria pinnatifida as an inductive substance and activities of lysozyme,superoxide dismutase(SOD) and catalase(CAT) in the lymph were assayed of 7 days and 9 days after administration.The results showed that there were higher activities of lysozyme,SOD and CAT in the fish injected with the lectin than those in the fish in the control group.However,the activity of the lysozyme decreased from 18.83% to 12.69% from 7 days to 9 days after administration,meanwhile the activity of SOD increased from 66.67% to 139.99% and CAT increased from 16.60% to 63.63%,indicating that the lectin can improve nonspecific immunity in crucian carp.
Keywords:Undaria pinnatifida  lectin  Carassius auratus  immunity
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