| 光度法快速测定大豆水溶性蛋白质的研究 |
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| 引用本文： | 赵宇明,姜俊,张建宁,翟丽莉. 光度法快速测定大豆水溶性蛋白质的研究[J]. 大豆科学, 2010, 29(1) |
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| 作者姓名： | 赵宇明 姜俊 张建宁 翟丽莉 |
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| 作者单位： | 大连市产品质量监督检验所,辽宁,大连,116021;大连宇达新材料化工研究所,辽宁,大连,116012 |
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| 摘 要： | 在室温条件下的酸性介质中,大豆水溶性蛋白质能与偶氮洋红G(azocarmine G)迅速结合,生成紫红色复合物,该复合物的最大吸收波长为580 nm,比偶氮洋红G红移了50 nm,其吸光强度与大豆水溶性蛋白质的质量浓度在3~34μg.mL-1范围内存在较好的选择性和较宽的线性关系。依此建立了测定大豆水溶性蛋白质的新方法,同时优化了大豆水溶性蛋白质的提取过程。该方法具有快速、稳定的特点,与凯氏法所得测定结果基本一致。
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| 关 键 词： | 蛋白质 偶氮洋红G 水溶性蛋白质 分光光度法 |
Investigation on Rapid Measurement of Water-soluble Soybean Protein through Spectrophotometry |
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| ZHAO Yu-ming,JIANG Jun,ZHANG Jian-ning,ZHAI Li-li. Investigation on Rapid Measurement of Water-soluble Soybean Protein through Spectrophotometry[J]. Soybean Science, 2010, 29(1) |
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| Authors: | ZHAO Yu-ming JIANG Jun ZHANG Jian-ning ZHAI Li-li |
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| Affiliation: | 1.Dalian Institute of Product Quality Supervision and Inspection;Dalian 116021;2Dalian Yuda New-material Institute of Chemical Industry;Dalian 116012;Liaoning;China |
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| Abstract: | A purple-red complex could be formed rapidly between azocarmine G and water-soluble soybean protein in acidic medium.The absorbance of this complex had a maximum absorption wavelength at 580 nm and exhibited a bathochromic shift of 50 nm compared with the maximum absorption wavelength of azocarmine G.Its absorbance with the concentration of soluble proteins at least in 3～34 μg · mL~(-1) was linearly.A method on rapid measurement of water-soluble soybean protein was established based on above-mentioned.Simultaneously we optimized the extraction procedure of water-soluble soybean protein.The results obtained by this method was rapid,stable.And the results were same with that obtained by Kjeldahl's method. |
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| Keywords: | Protein Azocarmine G Water-soluble protein Spectrophotometry |
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