泸定百合过氧化氢酶(Ls-Cat1)基因的克隆及表达分析

过氧化氢酶(CAT)参与植物的多种胁迫反应。为探究百合中的CAT基因与百合抗镰刀菌病害的关系,根据百合尖孢镰刀菌(Fusarium oxysporum)诱导的泸定百合(Lilium sargentiae Wilson)SSH文库中筛选到的CAT基因中间序列片段,通过RACE结合RT-PCR技术从泸定百合中克隆到1个CAT基因全长c DNA序列,该基因全长1 743 bp,包含1个1 479 bp的开放阅读框,编码492个氨基酸,命名为LsCat1(Gen Bank登录号:KU355272)。序列比对分析表明,Ls-Cat1编码的氨基酸序列中包含已确定的CAT保守基序。系统进化树分析显示,Ls-Cat1与其它物种CAT基因编码的氨基酸序列具有较高的相似性。qPCR结果显示,Ls-Cat1经百合镰刀菌诱导后明显上调表达,推测该基因可能与百合抗镰刀菌病害相关。本研究结果为揭示百合对镰刀菌枯萎病的抗病分子机制提供了一定的理论依据。

Cloning and Expression Analysis of Catalase (Ls-Cat1) Gene in Lilium sargentiae Wilson

In plant,catalase (CAT) plays an important role in detoxification of active oxygen species that are generated by various environmental stresses.To investigate the relationship between CAT and disease resistance to Lily Fusarium wilt,a novel CAT Gene was cloned from L.sargentiae Wilson by using RACE and RT-PCR,according to the EST sequence in of CAT SSH library induced by F.oxysporum.The full length of cDNA sequence was 1 743 bp with a complete open reading frame (ORF),which encodes 492 amino acids,the gene was named as Ls-Cat1 (GenBank:KU355272).Amino acid sequence of the Ls-Cat1 was highly homologous with CAT genes of many plant species based on the results of alignment.qPCR analysis showed that the Ls-Cat1was up-regulated after infection by F.oxysporum.It was speculated that the Ls-Cat1 gene might play a certain role in the disease resistance reaction for Lily Fusarium wilt.The results of this research provided some valuable information for further disclosing the molecular mechanism of resistance to Lily Fusarium Wilt.