二次正交旋转组合设计优化牛肝菌蛋白提取工艺

为明确牛肝菌蛋白提取条件,以牛肝菌子实体为材料,碱溶液为提取剂,在单因素试验基础上,采用四因素五水平的二次正交旋转组合试验设计,研究碱浓度、浸提温度、浸提时间和液固比对蛋白质提取得率的影响,经统计分析,建立数学回归模型。结果表明,所得回归方程达到极显著水平,无失拟因素存在;通过频率分析法得到的牛肝菌蛋白质的最佳提取工艺为:Na OH浓度0.11mol·L~(-1),液固比31∶1,浸提温度62℃,浸提时间1.70 h,此条件下蛋白质实际提取得率为19.85%。本试验优化牛肝菌蛋白的提取工艺参数准确可靠、稳定、可行,为牛肝菌蛋白功能的进一步研究和食用菌功能性食品开发提供了一定的技术依据。

Optimization of Extraction Technology for Protein From Boletel by Quadratic Regression Rotation-orthogonal Combination Design

In order to study the the extraction conditions of protein from boletel,on the basis of single-factor test,four factors quadratic orthogonal rotation design was used to investigated the factors (alkali concentration,extraction temperature,extraction time and liquid-solid ratio) influencing the yields of protein from boletel.And the mathematical regression model was established through stat analysis.The results showed that the obtained regression equation reached to significant level without any lack of fit factor.The optimum extraction conditions of protein from boletel were as follows:NaOH concentration 0.11 mol· L-1,liquid-solid ratio31 ∶ 1,extraction temperature 62℃,extraction time 1.70 h.Under these conditions the protein yield was 19.85％.The extraction parameters of protein optimized were accurate,stable,feasible,and provided a theoretical basis and technical support for the further study of protein from boletel.